SYBR Green Real-Time PCR Master Mixes

SYBR Green dye-based master mixes for real-time PCR

When using dye-based chemistry for real-time PCR analysis, master mix choice matters. As the pioneers of SYBR Green dye quantification for qPCR, we have evolved our SYBR Green master mix formulations over many years into highly consistent reagents that deliver exceptional sensitivity and specificity on any qPCR instrument.

Compare SYBR Green vs TaqMan chemistries for qPCR analysis

SYBR Green master mix products

For optimal performance using SYBR green dye-based qPCR, we recommended using our latest formulation, PowerTrack SYBR Green Master Mix. For automated workflows that require 72-hour bench-top stability, we recommend PowerUP SYBR Green Master Mix.

PowerTrack SYBR Green Master Mix

PowerTrack SYBR Green Master Mix boosts reaction setup accuracy with an optional visual-tracking dye and simplifies primer optimization by accommodating a broad primer Tₘ range.

PowerUP SYBR Green Master Mix

PowerUp SYBR Green Master Mix features 72-hour benchtop stability with a dual hot-start mechanism for excellent specificity.

Compare PowerTrack and PowerUp SYBR Green master mixes

 

PowerTrack SYBR Green Master Mix

PowerUp SYBR Green Master Mix

Chemistry

2-step SYBR Green dye

 2-step SYBR Green dye

Target input

cDNA

 cDNA

Concentration

2X

 2X
Polymerase Taq DNA Polymerase Dual-Lock Taq DNA Polymerase
Multiplexing and passive reference dye  With ROX: 1 target With ROX: 1 target

Cycling mode: run time

Fast: ~30 min

Standard: ~60 min

Fast: ~30 min

Standard: ~60 minutes
Sample tracking dye (optional) Yes No
Reaction benchtop stability 8 hours 72 hours

Carryover contamination control

With UNG

With UNG

 

Reverse transcriptase

(sold separately)

 

SuperScript IV Vilo Master Mix

 High-Capacity RNA-to-cDNA™ Kit

Assay recommendations

Sequence detection primers

 Sequence detection primers

Ordering information

Quick order below or visit product page

 Visit product page

Other SYBR Green Master Mixes

Our legacy SYBR Green master mixes, including Power SYBR Green PCR Master Mix, SYBR Green PCR Master Mix, Fast SYBR Green qPCR Master Mix, and SYBR Select Master Mix have long supported researchers with their qPCR analysis. However, for optimal results we recommend upgrading to our newest master mixes, which offer superior performance and compatibility with any qPCR instrument.

Master mix performance data

For reliably accurate qPCR data, our latest formulation, PowerTrack SYBR Green Master Mix, has been optimized to provide highly specific target amplficiation and excellent data reproducibilty over a wide dynamic rage.

Figure 1. Highly specific amplification

Real-time PCR was performed using universal human reference (UHR) cDNA and primers targeting PGK1 (phosphoglycerate kinase 1), ARL1 (ADP-ribosylation factor-like protein 1), and SNF8 (vacuolar-sorting protein). Reactions were run in quadruplicate using master mixes from different suppliers. PowerTrack SYBR Green Master Mix generated a single melt curve in 100% of the reactions, indicating specific target amplification.

Figure 2. Excellent data reproducibility

PowerTrack SYBR Green Master Mix shows reproducibility over a wide dynamic range. Six assays (PGK1, ARL1,SNF8, DF, GAPDH, and Corf1 19) were run in quadruplicate with UHR cDNA generated from four different RT kits. Assays were run with a 6-fold dilution series and 400 nM primer concentration.

Instrument compatibility

Applied Biosystems SYBR Green master mixes can be used with any real-time PCR instrument, from any manufacturer. Refer to your master mix user guide for recommended experimental run conditions.

Ordering information

Quickly order PowerTrack SYBR Green Master Mix below. To purchase other SYBR Green master mixes, visit the product page for ordering information.

 

For Research Use Only. Not for use in diagnostic procedures.

1x1 image pixel for data collection