Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Référence | Quantité |
---|---|
4309155 | 1 x 5 ml |
4344463 | 1 x 1 ml |
4364344 | 2 x 5 ml |
4364346 | 5 x 5 ml |
4312704 | 10 x 5 ml |
4334973 | 1 x 50 ml |
SYBR Green PCR Master Mix is not supported for use with Fast Mode thermal cycling conditions. When using SYBR Green PCR Master Mix on the StepOne, 7500 Fast, or 7900HT Fast instruments, use Standard mode thermal cycling conditions.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
Yes. On the Detector Manager for the System, set the Quencher to “Non Fluorescent”.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
No. We do not recommend that you use AmpErase UNG when performing reverse transcription. When using a dNTP mix with dUTP in a RT reaction, uracil will get incorporated into the cDNA generated from your RNA template. AmpErase UNG (uracil N-glycosylase), through an enzymatic reaction, will cleave single or double stranded DNA dUTP containing sequences.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
The Power SYBR Green PCR Master Mix is a new and improved formulation of the regular SYBR Green PCR Master Mix. You may need to verify your assays in order to start using the Power SYBR Green PCR Master Mix. For more information on verifying your reaction with the Power SYBR Green PCR Master Mix, please refer to the Power SYBR Green PCR Master Mix protocol.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
SYBR Green PCR Master Mix (P/N 4309155) does not contain AmpErase UNG. It contains the following components: SYBR Green 1 dye, AmpliTaq Gold DNA polymerase, dNTPs (with dUTP), Passive Reference 1 and optimized buffer components; all the necessary components to perform real-time PCR in a convenient premixed 2X solution. This product has been tested on and is supported for use on an ABI PRISM Sequence Detection System. For more information, please consult the SYBR Green PCR Master Mix protocol.
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