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DNA Polymerases
Shop from a wide variety of DNA polymerases also known as PCR enzymes. Our enzymes are available in stand-alone and master mixes formats. The stand-alone enzymes give you maximum flexibility in designing your assays, while our master mixes contain all the necessary reaction components in optimized concentrations for ready-to-use convenience. PCR enzymes facilitate polymerase chain reactions (PCR) that has wide ranging applications including DNA cloning, gene expression analysis, genotyping, mutation detection, forensics, pathogen detection, DNA sequencing, environmental monitoring, and molecular diagnostics. Our PCR enzymes have high fidelity, processivity, and sensitivity. Our hot-start polymerases are engineered to provide high specificity and sensitivity by minimizing non-specific amplification. Our direct master mixes amplify target DNA sequences from crude samples. The various formats offer product capabilities such as universal annealing, multiplexing, benchtop stability, and inhibitor tolerance. Learn more about DNA polymerases.
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DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities
Platinum™ Tfi DNA Polymerase
DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.
Catalyzes polymeration of deoxyribonucleotides into DNA strands. Suitable for PCR and automated sequencing reactions This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand.
T4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities
T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'≥3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'≥5' exonuclease activity towards single- and double-stranded DNA.
DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity
An optimized mixture of both enzymes for efficient nick translation of DNA
dsDNase is a heat inactivatable recombinant shrimp DNase used to remove genomic DNA contamination of RNA samples before reverse transcription.
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DNA labeling by nick-translation Second strand cDNA synthesis DNA blunting by fill-in 5'- overhangs Random-primed DNA labeling Labeling by fill-in 5'- overhangs of dsDNA DNA sequencing Site-specific mutagenesis Second strand cDNA synthesis Random-primed DNA labeling Labeling by fill-in 5'- overhangs...
Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR yield and specificity. AccuPrime technology is used in Taq , Pfx , and GC-rich formulations., The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of...
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Tfi DNA Polymerase
Can EquiPhi29 DNA Polymerase be heat inactivated?