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Slide Mounting Media
Browse through our collection of slide mounting media, designed to facilitate the mounting of tissue specimens or samples on slides for microscopic examination. These products include antifade mountants, coverslip sealants, live antifade reagents, and mounting mediums. Available in various formulations, slide mounting media can be found with or without added staining agents like DAPI for nuclear counterstaining. Antifade mountants, such as ProLong and SlowFade, are designed to protect fluorescent dyes and proteins from photobleaching, allowing for long-term imaging experiments. Some mounting media are specifically formulated with a refractive index optimized for high-resolution microscopy. Glycerol-based options offer both hard-setting and non-curing varieties, catering to different preservation needs. These products are crucial for researchers in fields such as histology, cytology, and fluorescence microscopy, enabling clear and lasting sample visualization. Learn more about antifades and mounting media .
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When sample thickness and imaging depth is not of concern, ProLong Diamond Antifade Mountant provides superior photobleach protection for Alexa Fluor dyes and fluorescent proteins allowing long term imaging experiments without loss of signal.
ProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image.
ProLong Glass Antifade Mountant provides sample and fluorescence preservation but also exhibits the same RI as glass and immersion oil (1.52) allowing high resolution microscopy imaging of cell and tissue samples up to 150 microns in thickness.
ProLong Diamond provides superior photobleach protection to Alexa Fluor dyes and fluorescent proteins for long term imaging experiments without loss of signal. With the added benefit of DAPI within the mountant, ProLong Diamond Antifade Mountant with DAPI makes multiplexing smooth and effortless.
SlowFade™ Diamond Antifade Mountant
Designed for use in mounting slides following immunofluorescent staining. Water-soluble Fluoromount-G™ is used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2° to 8°C.
SlowFade Glass Antifade Mountant is a glycerol-based, ready-to-use, non-curing mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides.
ProLong Glass Antifade with NucBlue Stain contains the nuclear counterstain Hoechst 33342 to detect DNA and provides sample and fluorescence preservation. ProLong Glass exhibits the same RI as glass and immersion oil (1.
ProLong Coverslip Sealant seals the edges of the coverslip when samples are mounted between microscope slide and coverslip. It is designed specifically for use when slides are mounted using an antifade mountant, such as ProLong Antifade Mountant or Slowfade Antifade Mountant.
ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image.
SlowFade Glass Antifade Mountant is a glycerol-based, ready-to-use, non-curing mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides.
Prolong Live Antifade Reagent helps prevent the loss of fluorescent signal (photobleaching) during live cell imaging.
For use in mounting slides following immunofluorescent staining as well as staining double stranded DNA. Water-soluble Fluoromount-G™ is used to mount slides in which the final step of staining is aqueous
ProLong RapidSet Antifade Mountant is an advanced, hard-setting mounting medium designed to expedite the imaging process for fluorescently labeled biological samples. This aqueous two-component system hardens in less than one hour, enabling rapid preparation times without compromising imaging...
SlowFade™ Diamond Antifade Mountant with DAPI
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Researchers typically use a mounting medium to maintain their samples good condition, either for a short period or for long-term storage and preservation. Learn about the basic types of mounting media and what to consider when choosing one so that you gain the level of protection and preservation...
Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity, particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration.
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Which mounting media can I use with the Nunc Lab-Tek Chamber Slide System products that are made of Permanox plastic?
Technical Bulletin: Compatibility of Mounting Media on Nunc Permanox Slides