Enzymes and Inhibitors

Purified from bovine pancreas

Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone

Lambda DNA digested with EcoRI, 0.7% agarose, 5 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in a single buffer.

Lambda DNA, 0.7% agarose, 2 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Agarase specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides. Agarase allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose. The recovered nucleic acids can be directly used for amplification, cloning, sequencing, etc.

Lambda DNA digested with HindIII, 0.7% agarose, 6 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity

Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.

Lambda DNA digested with TaiI, 1.4% agarose, 143 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Lambda DNA digested with NcoI, 0.7% agarose, 4 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.