Human Corneal Epithelial cells treated with Chloroquine to induce autophagy

Gibco® Human Corneal Epithelial cells were treated with 30uM Chloroquine overnight. The following day the cells were fixed and permeabilized and labeled with 0.5ug/mL anti-LC3B with a goat anti mouse Alexa Fluor® 488 secondary (Green) Cells were counter stained with 1ug/mL Hoechst 33342 (Blue) Images were acquired with a Molecular Devices ImageXpress High content imager

Gibco® Human Corneal Epithelial cells were treated with 30uM Chloroquine overnight. The following day the cells were fixed and permeabilized and labeled with 0.5ug/mL anti-LC3B with a goat anti mouse Alexa Fluor® 488 secondary (Green) Cells were counter stained with 1ug/mL Hoechst 33342 (Blue) Images were acquired with a Molecular Devices ImageXpress High content imager

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