HPLC Maintenance Videos

Replace the needle or sample loop if either one becomes clogged or damaged. The needle seat should be replaced whenever it is worn out.

Required parts:

• New sample loop
• New needle unit
• New needle seat
• Screwdriver Torx T10

Replace the pump head if the wetted surface is damaged, resulting in internal leaks compromising pump performance or if the pressure sensor is faulty.

Required parts:

• New pump head
• Spacing tool
• Size 6 hexagonal wrench
• ¼” wrench

Preparation steps:

1. Flush the pump head with an appropriate solvent using the purge function
2. Turn off the pump flow and wait until the system pressure is zero

Replace the piston seals of your pump on a regular basis or whenever excessive seal leakage leads to retention time shifts and increased baseline noise. Replace the seal wash seals whenever you remove the pump head. You need not replace the support ring when you replace a piston seal. Replace the support ring only when applicable.

Required parts:

• New piston seals
• New seal wash seals
• Seal handling tool
• Syringe filled with isopropanol

Preparation steps:

1. Remove the pump head (see “How to replace the pump head”)
2. Remove the pistons
3. Remove the seal wash plate from the pump head

Replace the check valves of your pump whenever they are worn or damaged or you observe a leak.

Required parts:

• New check valve cartridges
• New check valve nuts (only in case of obvious damage)
• Size 13 open-end wrench
• Tweezers

Preparation steps:

1. Flush the pump head with an appropriate solvent using the purge function
2. Turn off the pump flow and wait until the system pressure is down to zero

If you see that the flow cell of your detector is damaged or leaking, it should be replaced.

Required parts:

• New flow cell

Preparation steps:

1. Flush the flow cell with isopropanol
2. Wait until the flow cell has cooled down

Replace the UV lamp when the recommended operating hours are exceeded, the lamp intensity is too low, the lamp intensity fluctuates, or you see other signs that the lamp may be defective.

Required parts:

• New UV lamp

Preparation steps:

1. Turn off the UV lamp
2. Turn off the main power switch to the detector
3. Disconnect the power cord
4. Wait until the UV lamp has cooled down