Online and downloadable materials for the Bigfoot Cell Sorter

 

The manuals, brochures, white papers, downloads, webinars, videos, and other resources in this section can help you get the most out of your Bigfoot Cell Sorter.

 

Tools

FREE—Flow Cytometry Panel Design Service and Panel Builder Tool

Our scientists can design panels for you as many times you need—free of charge, no product purchase needed.

Fluorescence Spectra Viewer

Assess the spectral compatibility of dyes and probes for experiments that utilize fluorescence detection techniques.

Quick guides to fluorophore and reagent selection

Select the right fluorophores and reagents for your application with these quick guides.

Acoustic focusing flow cytometry article

Flow and Sorting Article Collection

Get our latest in flow cytometry and cell sorting innovation, through these collection of articles.

 

 

Documentation and literature

Bigfoot User Group meeting presentations

39:33

The Development and Future of High Content Immune Analysis Presented by Pratip K. Chattopadhyay, PhD, Founder & CEO, Talon Biomarkers

From Imagination to Implementation: The development and future of high content immune analysis presented by Pratip Chattopadhyay at the Bigfoot Meeting.
27:27

Bigfoot at Babraham Institute Presented by Rachael Walker, Head of Flow Cytometry, Babraham Institute

Bigfoot @ Babraham presented by Rachael Walker from Babraham Institute at the Bigfoot User Group Meeting.
24:01

Tips and Tricks: Sorting for Single-Cell Genomics on Bigfoot Presented by Patricia Rogers, Associate Director, The Broad Institute

Single Cell Genomics presented by Patricia Rogers from Broad Flow Cytometry Facility at the Bigfoot User Group Meeting.
7:12

Cell Viability, Proliferation and Function after Single-Cell Sorting Presented by Matt Meyer, Staff Engineer, Systems Design, Thermo Fisher Scientific

Cell Viability, proliferation and function after single cell sorting as characterized by flow cytometry and imaging presented by Matt Meyer.

Webinars

55:58

Identifying and sorting elusive cells using a high dimensional spectral panel

Using a 28-color mouse immunophenotyping panel we demonstrate the spectral unmixing and sorting capabilities of the Bigfoot Spectral Cell Sorter to resolve fluorescent signals accurately and precisely while sorting six ways simultaneously with high e
22:37

Sorting it out: A comprehensive webinar on single-cell flow cytometry

Product manager Angie Goldfain presents on the basics of using flow cytometry and cell sorting for single-cell analysis.
1:02:56

Exploring the five pillars of cell sorting: Viability, recovery, efficiency, purity and proliferation

R&D scientist Matthew Meyer presents on key points for successful sort.
37:08

The Bigfoot Spectral Cell Sorter: Accelerating Science

Three flow cytometry core managers describe their experience with the speed, sensitivity, ease of use, and flexibility of the Bigfoot Spectral Cell Sorter during a panel at CYTO 2021.
7:00

Proper Controls for the Bigfoot Spectral Cytometer: Beads Are Not Cells; But Are They Close?

In this on demand webinar we will discuss what controls are necessary, the features of a good control sample, and how and when bead-based controls are appropriate on the Bigfoot platform.
8:10

Sorting large particles at low pressure on the Bigfoot Spectral Cell Sorter

The 200 µm nozzle tip was developed for the Invitrogen Bigfoot Spectral Cell Sorter for large cell sorting.
48:11

Isolation, detection, and analysis: Comprehensive strategies for exosome and extracellular vesicle research with automation enablement

This webinar explores workflow capabilities from exosome enrichment to separation detection and analysis with various techniques allowing for flexibility of application, sample throughput, and target size, and analysis.

Videos

1:47

Invitrogen Bigfoot Cell Sorter - Biosafety

An integrated biosafety cabinet helps ensure the safety of users and samples and is ergonomically optimized for sorting workflows. The adjustable sash can be raised or lowered for safe access to the sample area or sorting nozzles.
3:43

Invitrogen Bigfoot Cell Sorter - Overview

This video highlights the simpler workflow, spectral unmixing, high throughput, multiway sorting, improved biosafety, automation, and ease of use features of the Bigfoot Spectral Cell Sorter.
0:00

Invitrogen Bigfoot Cell Sorter - Spectral Unmixing

Specialized, state-of-the-art hardware allows fast real-time unmixing and high-purity spectral sorting. With no electronic aborts and index sorting always enabled, every cell is tracked in all sort modes from laser interrogation to the sort or abort location.
0:00

Invitrogen Bigfoot Cell Sorter - Ease of Use

The software supports quick startup, autocalibration, and accurate quality control. An experiment designer and one-button shutdown make the system easy to use and reduces downtime.
0:00

Invitrogen Bigfoot Cell Sorter - Time Savings and Higher Throughput

Sorting on Bigfoot Spectral Cell Sorter is exceptionally fast and offers an array of output options.
0:00

Invitrogen Bigfoot Cell Sorter - Swap tip wizard

Simply unscrew the retaining collar to remove and replace a nozzle tip with a new tip or one of a different size. After replacing the retaining collar, the swap tip wizard makes automatic QC adjustments to optimize the stream position.
0:00

Imagine Spectral Possibilities

Video expresses how much more information you can get from a cell using high parameter/spectral flow cytometry.

Customer stories

“The tech support experience is probably the best I’ve encountered in any of my work. The instrument itself is a pleasure to work with, and it’s fun to watch people new to flow in my lab take to it quickly and naturally. I’m really proud of the science we’re doing here—working in brain cancer, autoimmunity, leukemia, Alzheimer’s, and HIV—and so glad that it’s powered by the Bigfoot Spectral Cell Sorter and your team. I am so happy with the experience.” 

Pratip Chattopadhyay,

CEO, Talon Biomarkers

"We welcomed the Bigfoot Sorter to Babraham Institute in late August 2020, and it soon became apparent that the Bigfoot Sorter was going to be a key piece of technology for our core facility. The designers of the Bigfoot engaged with expert core managers when designing the machine and this certainly shows now it’s in the lab. The machine is designed flexibly for a core facility. In fact, I call it the Swiss army knife of cytometers….

 

Automation is key for us. The automated startup, alignment, and QC on the Bigfoot Sorter is amazing—17 minutes from start to finish.… Automated startup saves us at least an hour a day. That is an hour’s more sorting that I can charge for in my core facility. That equates to 20 hours a month more capacity and more income into my facility…. The Bigfoot Sorter can run for hours with little intervention….

 

The Bigfoot Sorter has been built for the core facility. It’s quiet and it’s bijou. The machine is so incredibly quiet that sometimes we have to question whether it’s on and the hood is working. But we know it is! This makes working on the machine a dream, especially when you compare this to the long sorts my team have to do with ear defenders on whilst using other sorters which have separate hoods. What has impressed us most at Babraham is the size of the machine and its integrated hood. In fact, Bigfoot has a small footprint.”

Rachael Walker, Head of Flow Cytometry

Babraham Institute, Babraham, Cambridgeshire, England

Educational resources

Educational resources for the Bigfoot Spectral Cell Sorter include an abundance of online materials on flow cytometry, fluorescence, and spectral analysis.

Flow Cytometry Learning Center

Among the many assets in our free online learning center you can find Spectral Flow Cytometry Fundamentals and Panel Design and Multicolor Flow Cytometry.

Molecular Probes School of Fluorescence

Developed by our in-house bench scientists, this guided learning is designed to help you rapidly learn the basics of fluorescence, imaging, and flow cytometry.

Visuals of e-signature workflows in both iBright Analysis Software-Secure and iBright Imaging System

Flow Cytometry Fundamentals

This guided learning module contains an introduction to flow cytometry and explains how the optics, fluidics, and electronics inside a flow cytometer work together to generate data from a flowing stream of labeled cells.

Immunology at Work Resource Center

Access rich and reliable technical content designed for new and experienced life scientists alike. Practical guidance and protocols will help you get started quickly. Of particular interest is the Foundational Immunology section characterizing different types of immune cells.

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Register your Bigfoot Spectral Cell Sorter to receive notifications of software and firmware updates.

For Research Use Only. Not for use in diagnostic procedures.