Oncomine Comprehensive Assay v3

Explore the Oncomine Comprehensive Assay v3

The Ion Torrent Oncomine Comprehensive Assay v3 on the Genexus System will enable you to:

Rapidly analyze relevant SNVs, indels, CNVs, and gene fusions from 161 genes. This allows you to report relevant biomarkers for key cancer types, including breast, colorectal, lung, ovarian, prostate, and others as soon as the next day and integrate them with immunohistochemistry results.

Save your technicians’ time with a highly automated workflow, offering a hands-on time of as little as 20 minutes.

Reduce complexity and cost of implementation and maintenance by utilizing a true end-to-end solution, from sample to report, without the need for additional bioinformatics expertise.

Generate results from 20 ng of DNA and 20 ng of RNA, enabling you to obtain results from small and low-quality FFPE samples, such as NSCLC or prostate cancer samples.

On Demand Webinar: Evaluation Of Oncomine Comprehensive Assay v3

Watch our OCA v3 webinar to discover how the Genexus System enables rapid NGS testing, featuring Dr. Bin Wang from SydPath-St. Vicent's Hospital in Sydney, Australia.

Bin Wang, MBBS, M.Med, Ph.D

Senior Hospital Scientist

SydPath–St. Vincent’s Hospital

Sydney, Australia

Bin Wang

End-to-end ngs solution

With only two touchpoints and as little as 20 minutes of hands-on time, the Oncomine Comprehensive Assay v3 on the Genexus System reduces laboratory staff burden and the potential for human errors and alleviates the need for specialized bioinformatics expertise by automating nucleic acid extraction and quantification, library preparation, sequencing, and analysis.

 

The Oncomine Comprehensive Assay v3 can start from FFPE on the Genexus Purification System or from purified nucleic acid on the Genexus Integrated Sequencer. Types of runs include DNA- or RNA-only, or DNA and RNA simultaneously.

ngs workflow

NGS systems

The Oncomine Comprehensive Assay v3 is available on the Genexus System and additionally available for use on the Ion GeneStudio S5 System with manual or automated library preparations.

Relevant biomarkers in precision oncology research across multiple tumors

The Oncomine Comprehensive Assay v3 covers the most relevant biomarkers for precision oncology research. This includes SNVs, indels, CNVs, and gene fusions from 161 genes encompassing cancer drivers and tumor suppressors. A subset of genes included in the panel are shown in the figure.

 

As DNA damage repair genes are becoming particularly relevant in multiple cancers including breast, ovarian, prostate, and pancreatic, the Oncomine Comprehensive Assay v3 includes genes in the homologous recombination repair (HRR) pathway, including ATM, BRCA1, BRCA2, CDK12, CHEK1, CHEK2, PALB2, RAD51B, RAD51C, and RAD51D, among others, enabling simultaneous analysis of HRR genes using a single workflow.

biomarker diagram

Gene coverage

Hotspot genes Full-length genes Copy number genes Gene fusions (inter-and intra-genetic)
AKT1 FGFR4 MED12 SRC ARID1A PALB2 AKT1 MDM2 AKT2 NF1
AKT2 FLT3 MET STAT3 ATM PIK3R1 AKT2 MDM4 ALK NOTCH1
AKT3 FOXL2 MTOR TERT ATR PMS2 AKT3 MET AR NOTCH4
ALK GATA2 MYC TOP1 ATRX POLE ALK MYC AXL NRG1
AR GNA11 MYCN U2AF1 BAP1 PTCH1 AR MYCL BRAF NTRK1
ARAF GNAQ MYD88 XPO1 BRCA1 PTEN AXL MYCN BRCA1 NTRK2
AXL GNAS NFE2L2   BRCA2 RAD50 BRAF NTRK1 BRCA2 NTRK3
BRAF H3F3A NRAS   CDK12 RAD51 CCND1 NTRK2 CDKN2A NUTM1
BTK HIST1H3B NTRK1   CDKN1B RAD51B CCND2 NTRK3 EGFR PDGFRA
CBL HNF1A NTRK2   CDKN2A RAD51C CCND3 PDGFRA ERBB2 PDGFRB
CCND1 HRAS NTRK3   CDKN2B RAD51D CCNE1 PDGFRB ERBB4 PIK3CA
CDK4 IDH1 PDGFRA   CHEK1 RB1 CDK2 PIK3CA ERG PPARG
CDK6 IDH2 PDGFRB   CREBBP RNF43 CDK4 PIK3CB ESR1 PRKACA
CHEK2 JAK1 PIK3CA   FANCA SETD2 CDK6 PPARG ETV1 PRKACB
CSF1R JAK2 PIK3CB   FANCD2 SLX4 EGFR RICTOR ETV4 PTEN
CTNNB1 JAK3 PPP2R1A   FANCI SMARCA4 ERBB2 TERT ETV5 RAD51B
DDR2 KDR PTPN11   FBXW7 SMARCB1 ESR1   FGFR1 RAF1
EGFR KIT RAC1   MLH1 STK11 FGF19   FGFR2 RB1
ERBB2 KNSTRN RAF1   MRE11A TP53 FGF3   FGFR3 RELA
ERBB3 KRAS RET   MSH2 TSC1 FGFR1   FGR RET
ERBB4 MAGOH RHEB   MSH6 TSC2 FGFR2   FLT3 ROS1
ERCC2 MAP2K1 RHOA   NBN   FGFR3   JAK2 RSPO2
ESR1 MAP2K2 ROS1   NF1   FGFR4   KRAS RSPO3
EZH2 MAP2K4 SF3B1   NF2   FLT3   MDM4 TERT
FGFR1 MAPK1 SMAD4   NOTCH1   IGF1R   MET  
FGFR2 MAX SMO   NOTCH2   KIT   MYB  
FGFR3 MDM4 SPOP   NOTCH3   KRAS   MYBL1  

Ordering information

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If you are interested in learning more about Oncomine CGP solutions for solid tumors and see a demo, please request contact from a local representative.

 

For Research Use Only. Not for use in diagnostic procedures.