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Phosphoramidites for Oligonucleotide Synthesis |
Thermo Scientific phosphoramidites are built to help meet varying needs of oligonucleotide synthesis from research applications to manufacturing. For research and early development, there are over 136 ready-to-ship phosphoramidites (abbreviated as amidites below) of small pack sizes in the online catalog.
Custom solutions and modification capabilities are available to support you from the beginning at the discovery phase. Our core scale-up competencies help ensure continuity all the way to the large-scale volume. With manufacturing expertise in custom nucleic acid chemistry for more than 40 years, our technical experts help ensure the consistent quality and security of supply chain for scaling up from grams to metric tons (MT).
Comprehensive supply of phosphoramidites
Amidites are the building blocks used in chemical synthesis of oligonucleotides. Optimize your oligo synthesis and manufacturing process development with our large selection of amidites offerings that are also available in two quality grades.
2’ Modified RNA phosphoramidites
Common base modifications—2′-MOE, 2′-OMe, and 2′-fluoro—for the synthesis of antisense oligos and siRNAs.
DNA phosphoramidites
For synthesis of DNA oligos used in research related to diagnostic and therapeutic development.
RNA phosphoramidites
Synthesis of RNA oligos for research in diagnostic and therapeutic development
Dye-labeled phosphoramidites
With fluorescent labels for synthesis of oligos for detection in molecular assays and microarrays.
Linkers and spacers
Linkers at 3′, 5′, or internal positions for NHS ester conjugation; and spacers to increase conformational support.
Structural phosphoramidites
With modifications (e.g., 5m-dC, N6-Me-dA, N4-Ethyl-dC, 8-oxo-dG) for synthesis of primers, probes, or oligos.
Succinates
DNA succinates for the derivatization of controlled pore glass, polystyrene, or other supports used in solid-phase oligo synthesis.
Oligonucleotide therapeutics
Custom services and product offerings to enable development from start to final commercialization.
Quality attributes of TheraPure chemistry solutions
Processes are monitored from starting material procurement to final QC and product release. Our rigorously controlled manufacturing processes help maximize batch-to-batch reproducibility and minimize levels of reactive P(III) impurities, which could accumulate as by-products into the oligonucleotide during synthesis.
| TheraPure | Standard | |
|---|---|---|
| Recommended uses |
|
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| Animal origin free (AOF) manufacturing | Manufactured with animal origin free (AOF) materials | |
| HPLC purity | 99% or higher with unknown, non-critical, and critical impurity specifications | 98% |
| 31P NMR purity | 99% or higher with unknown, non-critical, and critical impurity specifications | 98% |
| Water content | 0.3% or lower | |
| Solution clarity | 0.2 M solution in acetonitrile is free of undissolved particulates | – |
| UV spectral identification | The λ maxima of the sample and standard spectra are within ±2 nm | – |
| Residual solvents | 4.0% or lower | – |
Analytical data example: TheraPure Bz-dA phosphoramidite
Phosphoramidites are the key raw material and building blocks of oligonucleotides. Incorporating quality testing into the manufacturing process development from the beginning is a best practice for supporting research and development activities related to therapeutics or diagnostics. The comprehensive QC processes include the analysis of phosphoramidites that is crucial to monitoring the impurity levels.
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Typical 31P NMR scan of a TheraPure RNA phosphoramidite
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Impurity profile example of TheraPure 2ʹ-TBDMS-iBU-rG phosphoramidite by LC-MS
Manufacturing capabilities
Technical expertise
Over 40 years of expertise in custom chemistry and scale-up for innovative projects.
Security of supply
Increased capacity to serve both oligo therapeutics and diagnostic customers, all supported by a geographically diverse supply chain.
Refined quality
The site's Quality Management System (QMS) is certified to the ISO 9001 standard.
Quality manufacturing and documentation
Batch-to-batch consistency, minimal level of reactive impurities, and analytical documentation support.
Service excellence
48 hours or less response to orders, >98% delivery to customer at promised date*.
Flexible packaging
Multiple customization options for different oligo synthesizers, packaging configurations, and more.
*May not be available in all regions
Hear from the experts
Our scientists discuss the history of Thermo Fisher's Milwaukee facility, customer partnership, manufacturing process, quality improvement, mitigation of supply chain issues, and the recent response to global needs.
Quick tour of the Thermo Fisher Scientific facility at Milwaukee for phosphoramidites and nucleotides
Elevating product values with expertise, partnership, and commitment
Applications and manufacturing process of phosphoramidites and nucleotides
Improving and delivering quality in phosphoramidites
Custom services for oligonucleotide chemistry
In addition to our Thermo Scientific phosphoramidites, we offer custom services for oligonucleotide chemistry tailored to fit your needs in diagnostic and research applications. We are fully set up to provide customization of our existing phosphoramidites, synthesis of new compounds, and development of oligonucleotide delivery chemistries. Simply fill out this short form with your interest so that our representative can explore available options with you.
Advanced project management
- Customer involvement
- Dedicated cross-functional project team
- Project planning and execution tailored to each custom request
- Manufactured in a facility whose Quality Management System (QMS) is certified to the ISO 9001 standard
- Thoroughly documented processes provide consistent product quality with batch-to-batch consistency
Customization options
- Base or base protection modification
- Sugar modifications
- Changes to phosphitylation, or phosphitylation of custom molecules
- 5′ protection modification
Custom chemistries and capabilities
- Custom phosphoramidites, delivery chemistries, modified nucleotides, and reagents
- Modifiers and linkers
- Small scale (g) to large scale (MT) manufacturing
- Development of customized QC
- Custom filling, labeling, and packaging
 | Phosphoramidite customization options A. Dimethoxytrityl (DMT) substitution with alternate protecting groups B. Base = A, C, G, T, or U protection modifications or base modifications C. Sugar modifications and protection options |
TheraPure services—access to customized materials for producing nucleic acid therapeutics
Options for base protection groups and package formats
Depending on your selected phosphoramidites, they may be available in one or more of these options. Please contact us if you do not find one that meets your needs.
Base N-protections to choose depending on your deprotection methods, their sensitivity to base hydrolysis, and their reactivity with tags and labels:
| Package formats to accommodate your synthesis scales, synthesizers, and solvation methods:
| |
Related products
Oligonucleotide synthesis—chemical essentials
For customers involved in oligonucleotide synthesis, we offer custom chemicals specifically designed for your synthesis. These chemicals include extra-dry solvents, deblocking agents, CAP A, CAP B, activators, and oxidizers.
We understand that every oligo customer is different, and that’s why we offer chemicals and chemical blends formulated to your precise concentrations and tailored to meet your exact specifications.
Resources
Technical Spotlights
Custom Services and Quotation
Speak with a technical specialist about support available for the oligonucleotide manufacturing process development of your projects.
Phosphoramidite Learning Resource
Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.
mRNA Production Learning Library
Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.
For Research Use Only. Not for use in diagnostic procedures.
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