Pluripotent Stem Cell Protocol Handbook cover

At the onset of your stem cell research, we understand it can be daunting to know where to begin and what products are needed. The Pluripotent Stem Cell Protocol Handbook will provide you with the fundamental protocols, solutions, and resources needed to get you started in any workflow.

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Cell culture protocols

Mouse protocols

Reprogramming

Cell reprogramming protocols

Culture

Cell culture protocols

Mouse protocols

Engineering

Cell engineering protocols

Differentiation

Cell differentiation protocols

Ectoderm

Mesoderm

Endoderm

Additional differentiation protocols

Characterization

Characterization protocols

Featured stem cell 'how to' videos

Passaging reagents for PSC culture
This video provides an overview of different passaging reagents which can be used to accomplish varying degrees of dissociation of pluripotent stem cells.
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        1. Now Playing
          Up NextPassaging reagents for PSC culture

          This video provides an overview of different passaging reagents which can be used to accomplish varying degrees of dissociation of pluripotent stem cells.

        2. Now Playing
          Up NextSelecting a matrix for PSC culture

          This video will help you select a matrix to ensure success when culturing your pluripotent stem cells.

        3. Now Playing
          Up NextHow to cryopreserve pluripotent stem cells

          This video will provide step-by-step guidance on how to cryopreserve pluripotent stem cells (PSCs).

        4. Now Playing
          Up NextHow to prepare StemFlex Medium

          This video will provide step-by-step guidance on how to prepare complete Gibco™ StemFlex™ Medium for the feeder-free culture of pluripotent stem cells.

        5. Now Playing
          Up NextHow to prepare Essential 8 Medium for pluripotent stem cell culture

          This video will show you how to prepare complete Essential 8 Medium for the consistent, robust, cost-effective feeder-free culture of PSCs.

        6. Now Playing
          Up NextHow to adapt pluripotent stem cells from other PSC media into Essential 8 Medium

          This video will show you how to adapt PSCs adapted to feeder-based or feeder-free media to Essential 8 Medium and Vitronectin.

        7. Now Playing
          Up NextHow to passage pluripotent stem cells in Essential 8 Medium

          This video outlines simple passaging for feeder-free pluripotent stem cell culture with Essential 8™ Medium

        8. Now Playing
          Up NextHow to prepare vitronectin substrate plates for pluripotent stem cell culture

          This video will show you how to coat culture plates with vitronectin, so you will be ready to transfer newly forming iPSC colonies or frozen cultures to Essential 8 Medium.

        9. Now Playing
          Up NextHow to differentiate pluripotent stem cells (PSC) into cardiomyocytes

          This video provides an overview of the protocols used for differentiating human pluripotent stem cells in to beating cardiomyocytes using the Gibco PSC Cardiomyocyte Differentiation Kit. See how to get beating cardiomyocytes in three simple steps.  

        10. Now Playing
          Up NextHow to induce pluripotent stem cells in to definitive endoderm

          This video provides an overview of the protocols used for inducting human pluripotent stem cells in to definitive endoderm in just 48 hours using the Gibco PSC Definitive Endoderm Induction Kit. See how to get definitive endoderm cells in just 2 days

        11. Now Playing
          Up NextHow to use Gibco PSC Neural Induction Medium

          This video will show you how Gibco® PSC Neural Induction Medium generates up to 20 million neural stem cells from pluripotent stem cells in 7 days. Learn more at http://www.lifetechnologies.com/nim

        12. Now Playing
          Up NextHow to Use the TaqMan hPSC Scorecard Panel

          This video will show you how to prepare and run the TaqMan hPSC Scorecard Panel plates.

      For Research Use Only. Not for use in diagnostic procedures.