Mixed-Mode HPLC Columns

Overview

The column you need with multiple functionalities

Do you want to separate ionic and hydrophobic analytes in a single run? Separate polar, nonpolar, and ionizable compounds in a single analysis and achieve baseline separation of challenging polar analytes without derivatizing using a mixed-mode HPLC column. The stationary phases of Thermo Scientific mixed-mode columns combine reversed phase, ion-exchange, and/or HILIC selectivity. This eliminates the need for column switching, ion-paring, and using columns in series. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH, and solvent content to improve chromatographic separations and increase productivity.

Mixed-mode HPLC columns are well-suited for retaining ionic analytes, including pharmaceutical drugs and their counter ions. Mixed-mode liquid chromatography does not require the use of ion-pair reagents in the mobile phase, significantly improving mass spectrometry (MS) compatibility.

All HPLC and UHPLC columns

Acclaim Trinity P1 HPLC Columns Cation-exchange, anion-exchange, and reversed phase selectivity	Acclaim Trinity P2 HPLC Columns Cation-exchange, anion-exchange, and HILIC selectivity	Acclaim Trinity Q1 HPLC Columns Cation-exchange, anion-exchange, and reversed phase selectivity
Simultaneous separation of polar analytes and their counter ions with high efficiency Suitable for applications including: APIs and counterions Hydrophilic, monovalent ions Small pharmaceuticals	Separation of multivalent cations or anions and pharmaceuticals Suitable for applications including: Multivalent ions and APIs Hydrophilic ions Zwitterions Neutral sugars	Excellent separation of pharmaceuticals and their counter ions Suitable for applications including: Herbicides – diquat and paraquat Pharmaceutical QC
Download specification sheet View column selection	Download specification sheet View column selection	Download specification sheet View column selection

Acclaim Mixed-Mode WAX-1 HPLC Columns Reversed phase and anion-exchange selectivity	Acclaim Mixed-Mode WCX-1 HPLC Columns Reversed phase and cation-exchange selectivity	Acclaim Mixed-Mode HILIC-1 HPLC Columns Reversed phase and HILIC selectivity

Hydrophobic alkyl chain with an anion terminus Powerful selectivity for the separation of difficult mixtures of neutral and anionic analytes Suitable for applications including: Acids/organic acids Hydrophobic cations Hydrophilic anions Peptides Fat-soluble vitamins Pharmaceuticals	Hydrophobic alkyl chain with a carboxyl terminus Powerful selectivity for the separation of difficult mixtures of hydrophobic acids andneutral and cationic analytes Suitable for applications including Hydrophilic cations Hydrophobic anions Bases /Basic pharmaceuticals Aminophenols	Hydrophobic alkyl chain with a diol group at the terminus Provides adjustable selectivity for both polar and nonpolar molecules Suitable for applications including: Highly polar molecules Ethoxylated nonionic surfactants PEGS
Download specification sheet View column selection	Download specification sheet View column selection	Download specification sheet View column selection

Both our Thermo Scientific Acclaim Trinity column line and Thermo Scientific Acclaim Mixed-Mode column line are compatible with the Thermo Scientific Acclaim guard holder and coupler.

Mixed-Mode Liquid Chromatography – An Exciting Development in Separation Science

Discover the latest in mixed-mode liquid chromatography technology, learn about the unique chromatographic properties of mixed-mode columns, and explore analytical challenges that have been addressed by mixed-mode liquid chromatography in this webinar led by Dr. Joachim P. Weiss, Ph.D. and Serdar Bilgesoy.

View webinar

HPLC column selection guide

3 ways to search

Find an equivalent column to the one you’re using now

Find the best column for your USP method

Find the right column for your new method

Find your HPLC column with ease ›

Featured resources

Acclaim columns

Explore a wide variety of Acclaim columns to meet your laboratory's application needs with the Acclaim column selection guide.

Download technical note ›

Selectivity benefits

Exploring the dual retention mechanism of mixed mode Acclaim WAX-1 columns to tune selectivity in liquid chromatography.

Download white paper ›

Environmental

Acclaim Trinity P1 mixed-mode chromatography column coupled with an inert LC system: an innovative approach for underivatized highly polar pesticides separation.

Download technical note ›