Search Thermo Fisher Scientific
SYBR Select Master Mix
SYBR™ Select Master Mix offers advanced performance at an affordable price.
|
Ordering Information
SYBR Select Master Mix outperforms the competition with respect to:
- Specificity - specific amplification for 100% of assays
- Brightness - optimum brightness for high performance on most Real-Time PCR instruments
- Dynamic range - more tolerant to high input cDNA
- Sensitivity - true single copy detection as demonstrated with digital PCR
Exceptional specificity
Out of 44 assays and 3 independent replicate reactions only SYBR Select Master Mix had a single peak in all the melt curves (100% specificity).
Spectacular dynamic range and linearity
SYBR Select produces no NTC signal and is more tolerant to high-input DNA.
Click image to enlarge
Figure 3: Dynamic range and linearity shown for a 7-log dilution for PGK1 assay
Brightness that is just right
SYBR Select is brighter than the majority of competitor master mixes, but not too bright to upset some Real-Time PCR instruments.
Click image to enlarge
Figure 2: Average delta Rn (relative fluorescence) across 4 dilution points for PGK1 assay
Single copy detection
SYBR Select allows single copy detection using dPCR and gDNA with RNaseP target.
Click image to enlarge
Figure 4: The single copy RNase P gene was amplified using genomic DNA diluted to <1 copy per reaction. Then, a normal distribution of the CT values was obtained
Resources