Technical Resources

21010 - Basal Medium Eagle (BME), no Glutamine

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
L-Arginine hydrochloride	211.0	21.0	0.09952607
L-Cystine 2HCl	313.0	16.0	0.05111821
L-Histidine	155.0	8.0	0.051612902
L-Isoleucine	131.0	26.0	0.19847329
L-Leucine	131.0	26.0	0.19847329
L-Lysine hydrochloride	183.0	36.47	0.19928962
L-Methionine	149.0	7.5	0.05033557
L-Phenylalanine	165.0	16.5	0.1
L-Threonine	119.0	24.0	0.20168068
L-Tryptophan	204.0	4.0	0.019607844
L-Tyrosine disodium salt dihydrate	261.0	26.0	0.099616855
L-Valine	117.0	23.5	0.2008547
Vitamins
Biotin	244.0	1.0	0.0040983604
Choline chloride	140.0	1.0	0.007142857
D-Calcium pantothenate	477.0	1.0	0.002096436
Folic Acid	441.0	1.0	0.0022675737
Nicotinamide	122.0	1.0	0.008196721
Pyridoxal hydrochloride	204.0	1.0	0.004901961
Riboflavin	376.0	0.1	2.6595744E-4
Thiamine hydrochloride	337.0	1.0	0.002967359
i-Inositol	180.0	2.0	0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	200.0	1.8018018
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	97.7	0.81416667
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2200.0	26.190475
Sodium Chloride (NaCl)	58.0	6800.0	117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O)	138.0	140.0	1.0144928
Other Components
D-Glucose (Dextrose)	180.0	1000.0	5.5555553
Phenol Red	376.4	10.0	0.026567481

Reference:

1. Eagle, H. (1965) Proc. Exp. Biol. Med., 89:362.