Technical Resources

11765 - Ham's F-12 Nutrient Mix

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.07.50.1
L-Alanine89.08.90.099999994
L-Arginine hydrochloride211.0211.01.0
L-Asparagine-H2O150.015.010.10006667
L-Aspartic acid133.013.30.1
L-Cysteine hydrochloride-H2O176.035.120.19954544
L-Glutamic Acid147.014.70.1
L-Glutamine146.0146.01.0
L-Histidine hydrochloride-H2O210.021.00.1
L-Isoleucine131.04.00.030534351
L-Leucine131.013.10.1
L-Lysine hydrochloride183.036.50.19945355
L-Methionine149.04.50.030201342
L-Phenylalanine165.05.00.030303031
L-Proline115.034.50.3
L-Serine105.010.50.1
L-Threonine119.011.90.099999994
L-Tryptophan204.02.040.01
L-Tyrosine disodium salt dihydrate262.07.810.02980916
L-Valine117.011.70.1
Vitamins
Biotin244.00.00732.9918034E-5
Choline chloride140.014.00.1
D-Calcium pantothenate477.00.50.001048218
Folic Acid441.01.30.0029478457
Niacinamide122.00.0362.9508196E-4
Pyridoxine hydrochloride206.00.062.9126214E-4
Riboflavin376.00.0379.8404256E-5
Thiamine hydrochloride337.00.38.9020777E-4
Vitamin B121355.01.40.0010332103
i-Inositol180.018.00.1
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.033.220.2992793
Cupric sulfate (CuSO4-5H2O)250.00.00251.0E-5
Ferric sulfate (FeSO4-7H2O)278.00.8340.003
Magnesium Chloride (anhydrous)95.057.220.6023158
Potassium Chloride (KCl)75.0223.62.9813335
Sodium Bicarbonate (NaHCO3)84.01176.014.0
Sodium Chloride (NaCl)58.07599.0131.01724
Sodium Phosphate dibasic (Na2HPO4) anhydrous142.0142.01.0
Zinc sulfate (ZnSO4-7H2O)288.00.8630.0029965276
Other Components
D-Glucose (Dextrose)180.01802.010.011111
Hypoxanthine Na159.04.770.03
Linoleic Acid280.00.0842.9999999E-4
Lipoic Acid206.00.210.0010194174
Phenol Red376.41.20.0031880978
Putrescine 2HCl161.00.1610.001
Sodium Pyruvate110.0110.01.0
Thymidine242.00.70.002892562

Reference:

1. Ham, R. G. (1965) Proc. Nat. Acad. Sci., 53, 288.

Related Products:

21765 - Ham's F-12 Nutrient Mix
Cat No: 21765029 , 21765037