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Citations & References (9)
Gibco™
Ham's F-12 Nutrient Mix
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has sinceRead more
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| Catalog Number | Quantity |
|---|---|
| 11765047 | 1000 mL |
| 11765054 | 500 mL |
| 11765062 | 10 x 500 mL |
| 11765070 | 6 x 1000 mL |
Catalog number 11765047
Price (USD)
59.65
Online Exclusive
65.00Save 5.35 (8%)
Each
In stock
Quantity:
1000 mL
Price (USD)
59.65
Online Exclusive
65.00Save 5.35 (8%)
Each
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is available.
Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
Cell LineCHO, COS-7, and rat prostate epithelial cells
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeHam's F-12 Nutrient Mixture
Quantity1000 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3090845Certificate of AnalysisJun 05, 202511765047, 11765054
3138479Certificate of AnalysisMay 04, 202511765047, 11765054
3137971Certificate of AnalysisMar 15, 202511765047, 11765054
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (9)
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Citations & References
Abstract
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
Journal:J Biol Chem
PubMed ID:7730310
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type
Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.
Journal:J Biol Chem
PubMed ID:7665618
'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs
Identification of a minimum enhancer sequence for the type II collagen gene reveals several core sequence motifs in common with the link protein gene.
Journal:J Biol Chem
PubMed ID:8626777
'The type II collagen gene (Col2a1) is expressed primarily in chondrocytes. Transcription of Col2a1 is mediated by cell-specific regulatory elements located within the promoter and first intron. Here, we map a minimal enhancer and identify elements that determine cartilage-specific Col2a1 expression by analyzing the activity of a series of chimeric
Interaction between the components of the interferon gamma receptor complex.
Journal:J Biol Chem
PubMed ID:7673114
'Interferon gamma (IFN-gamma) signals through a multimeric receptor complex consisting of two different chains: the IFN-gamma receptor binding subunit (IFN-gamma R, IFN-gamma R1), and a transmembrane accessory factor (AF-1, IFN-gamma R2) necessary for signal transduction. Using cell lines expressing different cloned components of the IFN-gamma receptor complex, we examined the
Isolation, characterization, and differentiation of human multipotent dermal stem cells.
Journal:Methods Mol Biol
PubMed ID:23483399
Skin, as the body's largest organ, has been extensively used to study adult stem cells. Most previous skin-related studies have focused on stem cells isolated from hair follicles and from keratinocytes. Here we present a protocol to isolate multipotent neural crest stem-like dermis-derived stem cells (termed dermal stem cells or
9 total citations