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            • Primary Antibodies ›
            • CD90.2 (Thy1.2) Antibodies

            Invitrogen

            CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 450, eBioscience™

            20 Published Figures
            33 References
            View all (51) CD90.2 (Thy1.2) antibodies
            Datasheet
            Protocols
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            Catalog # 48-0902-82
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            Cite CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 450, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (20)
            Application
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 21

            CD90.2 (Thy-1.2) Antibody (48-0902-82) in Flow

            Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.015 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.0075 µg of Anti-Mouse CD90.2 (Thy-1.2) eFluor® 450 (right). Total viable cells were used for analysis.
            View Product

            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)
            CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)
            CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)
            CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

            Product Details

            48-0902-82

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (IHC)

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            -
            View 1 publication 1 publication

            Flow Cytometry (Flow)

            0.015 µg/test
            View 30 publications 30 publications

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            53-2.1

            Conjugate

            eFluor™ 450
            • Unconjugated
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 615
            • eFluor 506
            • FITC
            • NovaFluor Blue 510
            • NovaFluor Blue 555
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 760
            • NovaFluor Red 710
            • NovaFluor Yellow 570
            • NovaFluor Yellow 660
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Request custom conjugation

            Excitation/Emission Max

            405/445 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_1272200

            Product Specific Information

            Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

            Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

            Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

            View more View less

            Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

            View more View less

            UniProt ID: (Mouse) P01831

            View more View less

            Entrez Gene ID: (Mouse) 21838

            View more View less

            Function(s)

            GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding cell adhesion molecule

            Process(es)

            angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol
            It has to be done as per old AB suggested Products section.
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