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Application
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Mouse IgG (H+L) Secondary Antibody (A28175) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antib... View More
Product Details
A28175
Applications
Tested Dilution
Western Blot (WB)
1:2,500-1:5,000
Immunohistochemistry (Frozen) (IHC (F))
1:2,000
Immunocytochemistry (ICC/IF)
1:1,000-1:2,000
Flow Cytometry (Flow)
1-5 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Recombinant full-length protein
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Gravity column chromatography
Storage buffer
PBS
Contains
5mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2536161
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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