Invitrogen

HK2 Monoclonal Antibody (3D3)

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

5 Published Figures

7 References

View all (23) HK2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HK2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

HK2 Antibody (MA5-15679) in ICC/IF

Immunofluorescence analysis of HK2 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HK2 Monoclonal Antibody (3D3) (Product # MA5-15679) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More

HK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HK2 Antibody in Immunohistochemistry (IHC)

HK2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

HK2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inHK2 KO cell line compared to control cell line using HK2 Monoclonal Antibody (3D3) (Product # MA5-15679). {KO}

Product Details

MA5-15679

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3D3

Immunogen

Purified recombinant fragment of human HK2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10986812

Product Specific Information

MA5-15679 targets HK2 in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples.

The MA5-15679 immunogen is purified recombinant fragment of human HK2 expressed in E. Coli.

MA5-15679 detects HK2 which has a predicted molecular weight of approximately 102kDa.

Target Information

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hypoxia induces a glycolytic complex in intestinal epithelial cells independent of HIF-1-driven glycolytic gene expression.

Proceedings of the National Academy of Sciences of the United States of America

Kierans SJ,Fagundes RR,Malkov MI,Sparkes R,Dillon ET,Smolenski A,Faber KN,Taylor CT

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Western Blot

Tue Aug 29 00:00:00 EDT 2023

Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia.

Journal of bone oncology

Yang J,Liu Z,Liu B,Sun L

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunohistochemistry

Thu Dec 01 00:00:00 EST 2022

GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.

International journal of molecular sciences

Marwarha G,Røsand Ø,Slagsvold KH,Høydal MA

MA5-15679 was used in Enzyme-linked immunosorbent assay to unveil that the inhibition of GSK3β kinase activity is indispensable in mediating the miR-210-orchestrated protective cellular response to hypoxia-induced apoptotic cell death.

Fri Aug 19 00:00:00 EDT 2022

Higher Glucose Enhances Breast Cancer Cell Aggressiveness.

Nutrition and cancer

Santos JM,Hussain F

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Western Blot

Wed Apr 14 00:00:00 EDT 2021

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738

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Gene Aliases: HK2; HKII; HXK2

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UniProt ID: (Human) P52789

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Entrez Gene ID: (Human) 3099

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Function(s)

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity kinase

Process(es)

cellular glucose homeostasis response to ischemia glycolytic process lactation apoptotic mitochondrial changes glucose transport negative regulation of mitochondrial membrane permeability regulation of glucose import carbohydrate phosphorylation glucose 6-phosphate metabolic process canonical glycolysis establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of mitophagy in response to mitochondrial depolarization negative regulation of reactive oxygen species metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HK2 Monoclonal Antibody (3D3)

HK2 Antibody (MA5-15679) in ICC/IF

Product Details

MA5-15679

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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