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Application
FIGURE: 1 / 1
LNX1 Antibody (CF800747) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LNX1 (RC206386, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LNX1. Positive lysates LY403178 (100 µg) and LC403178 (20 µg) can be purchased separately from OriGene.
Product Details
CF800747
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1H9
Immunogen
Human recombinant protein fragment corresponding to amino acids 260-528 of human LNX1 produced in E.coli.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Lnx1 (Ligand of Numb Protein X 1) protein is encoded by the Lnx1 gene located on chromosome 4 in humans and is known as PDZRN2 (PDZ domain-containing RING finger 2). It is through its RING finger and PDZ domains that Lnx1 forms oligomers and interacts with some of its target proteins such as JAM4 to form multiprotein complexes. Lnx1 is best studied as a E3 ubiquitin ligase that also contains the NPXY motifs which allows binding to the Phosphotyrosine binding domain (PTB) on Numb protein. This interaction has been shown to be responsible for ubiquitination of Numb, which prevents the proteasomal degradation of Notch intracellular domain (NICD) and thus positively regulates the Notch signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase LNX; Ligand of Numb-protein X 1; ligand of numb-protein X 1, E3 ubiquitin protein ligase; multi-PDZ-domain-containing protein, E3 ubiquitin-protein ligase LNX; Numb-binding protein 1; PDZ domain-containing RING finger protein 2; RING-type E3 ubiquitin transferase LNX
Gene Aliases: LNX; LNX1; MPDZ; PDZRN2; UNQ574/PRO1136
UniProt ID: (Human) Q8TBB1
Entrez Gene ID: (Human) 84708
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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