Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
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AA Sequence of recombinant protein: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NNT-1/BCSF-3. Anti-Human NNT-1/BCSF-3-specific antibody was purified by affinity chromatography employing an immobilized Human NNT-1/BCSF-3 matrix.
Sandwich ELISA: To detect Human NNT-1/BCSF-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NNT-1/BCSF-3 (500-P186Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NNT-1/BCSF-3.
Western Blot: To detect Human NNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NNT-1/BCSF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
NNT-1/BCSF-3 is a neurotrophic factor with B-cell-stimulating capabilities. Expressed in the lymph nodes and spleen, NNT-1/BCSF-3 activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b) by binding and inducing the tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss, and B cell hyperplasia associated with increases in serum IgG and IgM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell stimulating factor 3; B-cell stimulatory factor 3; B-cell-stimulating factor 3; BSF-3; Cardiotrophin-like cytokine factor 1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT-1; Novel neurotrophin-1
Gene Aliases: BSF-3; BSF3; CISS2; CLC; CLCF1; NNT-1; NNT1; NR6
UniProt ID: (Human) Q9UBD9
Entrez Gene ID: (Human) 23529
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