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Product Details
66733-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1B3D7
Immunogen
RHOA Fusion Protein Ag1141 (1-193 aa encoded by BC005976)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MAAIRKKLV IVGDGACGKT CLLIVFSKDQ FPEVYVPTVF ENYVADIEVD GKQVELALWD TAGQEDYDRL RPLSYPDTDV ILMCFSIDSP DSLENIPEKW TPEVKHFCPN VPIILVGNKK DLRNDEHTRR ELAKMKQEPV KPEEGRDMAN RIGAFGYMEC SAKTKDGVRE VFEMATRAAL QARRGKKKSG CLVL (1-193 aa encoded by BC005976)
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aplysia ras-related homolog 12; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; h12; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; Rho; Rho A; Rho cDNA clone 12; Rho family GTPase; small GTP binding protein RhoA; Transforming protein RhoA
Gene Aliases: ARH12; ARHA; Arha1; Arha2; RHO12; RHOA; RHOH12
UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0
Entrez Gene ID: (Pig) 396849, (Human) 387, (Rat) 117273, (Mouse) 11848
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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