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Proteintech

RLIM Polyclonal Antibody

View all (10) RNF12 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (3)

Application

RLIM Antibody in Western Blot (WB)

FIGURE: 1 / 3

RLIM Antibody (16121-1-AP) in WB

Jurkat cells were subjected to SDS PAGE followed by western blot with 16121-1-AP (RLIM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

RLIM Antibody in Western Blot (WB)

RLIM Antibody in Western Blot (WB)

RLIM Antibody in Immunoprecipitation (IP)

Product Image Gallery

RLIM Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 16121-1-AP (RLIM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

RLIM Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 16121-1-AP (RLIM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RLIM Antibody in Immunoprecipitation (IP) — IP result of anti-RLIM (IP:16121-1-AP, 4ug; Detection:16121-1-AP 1:500) with HeLa cells lysate 1520ug.

Product Details

16121-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RLIM Fusion Protein Ag9077 (276-350 aa encoded by BC013357)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SGPELLSRG LFAASGTRNA SQGAGSSDTA ASGESTGSGQ RPPTIVLDLQ VRRVRPGEYR QRDSIASRTR SRSQTP (276-350 aa encoded by BC013357)

Target Information

RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis.

Cell death & disease

Huang Y,Liu S,Shan M,Hagenaars SC,Mesker WE,Cohen D,Wang L,Zheng Z,Devilee P,Tollenaar RAEM,Li Z,Song Y,Zhang L,Li D,Ten Dijke P

Mon Jan 10 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Journal of molecular cell biology

Li Y,Yang C,Wang H,Zhao L,Kong Q,Cang Y,Zhao S,Lv L,Li Y,Mao B,Ma P

Tue Apr 05 00:00:00 UTC 2022

RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21.

Oncotarget

Huang Y,Nie M,Li C,Zhao Y,Li J,Zhou L,Wang L

Tue Oct 10 00:00:00 UTC 2017

RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21.

Oncotarget

Huang Y,Nie M,Li C,Zhao Y,Li J,Zhou L,Wang L

Tue Oct 10 00:00:00 UTC 2017

PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.

Oncotarget

Li C,Zhou X,Chen J,Lu Y,Sun Q,Tao D,Hu W,Zheng X,Bian S,Liu Y,Ma Y

Tue Sep 29 00:00:00 UTC 2015

PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.

Oncotarget

Li C,Zhou X,Chen J,Lu Y,Sun Q,Tao D,Hu W,Zheng X,Bian S,Liu Y,Ma Y

Tue Sep 29 00:00:00 UTC 2015

MOF-associated complexes ensure stem cell identity and Xist repression.

eLife

Chelmicki T,Dündar F,Turley MJ,Khanam T,Aktas T,Ramírez F,Gendrel AV,Wright PR,Videm P,Backofen R,Heard E,Manke T,Akhtar A

Mon May 19 00:00:00 UTC 2014

MOF-associated complexes ensure stem cell identity and Xist repression.

eLife

Chelmicki T,Dündar F,Turley MJ,Khanam T,Aktas T,Ramírez F,Gendrel AV,Wright PR,Videm P,Backofen R,Heard E,Manke T,Akhtar A

Mon May 19 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CTD-2530H13.3; DKFZp686N06224; E3 ubiquitin-protein ligase RLIM; E3 ubiquitin-protein ligase RNF12; FLJ25923; FLJ41093; FLJ42887; FLJ45986; Hoxa1 regulated; LIM domain-interacting RING finger protein; MGC15161; R-LIM; Renal carcinoma antigen NY-REN-43; RING finger LIM domain-binding protein; RING finger protein 12; ring zinc finger LIM domain binding protein; ring zinc finger protein NY-REN-43antigen; RING-type E3 ubiquitin transferase RLIM

Gene Aliases: AL022832; AW743871; Ha1r; NY-REN-43; RLIM; Rlim1; RNF12

UniProt ID: (Human) Q9NVW2, (Mouse) Q9WTV7

Entrez Gene ID: (Human) 51132, (Rat) 317241, (Mouse) 19820

Function(s)

transcription corepressor activity ubiquitin-protein transferase activity zinc ion binding ligase activity ubiquitin protein ligase activity protein binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process protein ubiquitination negative regulation of sequence-specific DNA binding transcription factor activity random inactivation of X chromosome protein polyubiquitination transcription, DNA-templated regulation of transcription, DNA-templated proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of transcription, DNA-templated regulation of dosage compensation by inactivation of X chromosome

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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