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Application
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SPHK1 Antibody (PA5-28584) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of SPHK1 in paraformaldehyde-fixed HeLa. Samples were incubated with SPHK1 polyclonal antibody (Product # PA5-28584) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.
Product Details
PA5-28584
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human SPHK1. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546060
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Predicted reactivity: Human (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acetyltransferase SPHK1; RP23-193A16.4; SK 1; SKase; Sphingosine kinase 1; SPK 1
Gene Aliases: SK1; SPHK; SPHK1; SPK
UniProt ID: (Human) Q9NYA1
Entrez Gene ID: (Human) 8877
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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