SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV (Moloney Murine Leukemia Virus) RT mutant designed for reduced RNase H activity, increased thermostability, and highly efficient full-length cDNA synthesis. Compared to previous generation SuperScript RTs, SuperScript IV reverse transcriptase has significantly improved processivity, which enables fast reaction speed, inhibitor resistance, and exceptional performance even with challenging RNA samples. SuperScript IV reverse transcriptase is available in multiple formats tailored to specific applications, and it is widely cited in peer reviewed journals.

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Advantages of SuperScript IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase is known for its efficiency, sensitivity, robustness, short-reaction time, and thermostability. Click on the attributes below to see supporting data. For recommendations on choosing the reverse transcriptase fit for your application, use the selection tool.

  • Efficient—Up to 100x higher cDNA yield
  • Sensitive—Ct values reduced by as many as eight cycles for RT-qPCR
  • Robust—Transcribes degraded and inhibitor-containing RNA samples
  • Fast—10-minute cDNA synthesis time
  • Stable—High thermostability to transcribe structured templates

 Read white paper to see data

SuperScript IV Reverse Transcriptase formats: Selection guide

SuperScript IV RT is available in several formats (Table 1)

  • Stand-alone enzyme—Reverse transcriptase supplied with reaction buffer
  • First-strand synthesis system—All cDNA synthesis reaction components are in separate tubes for maximum flexibility of reaction conditions
  • Master mix—cDNA synthesis reaction components are premixed for exceptional efficiency and reduced variability in RT-qPCR applications
  • One-step RT-PCR system—RT and PCR reagents for one-step RT-PCR applications
  • Direct reverse transcription—Reagents for cell lysis and cDNA synthesis included; RNA purification is not required

Table 1. SuperScript IV RT formats.

ProductApplications
Optimal reaction temperatureRT reaction timeAvailable formats

SuperScript IV Reverse Transcriptase stand-alone enzyme

SuperScript IV Reverse Transcriptase

Most effective enzyme for all types of RNA, including difficult templates50–55°C10 minStandalone enzyme
Order now

Product photo. Package and five tubes of kit components.

SuperScript IV VILO Master Mix

First-strand cDNA synthesis reaction mix for two step RT-qPCR50°C10 min

SuperScript IV VILO Master Mix
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SuperScript IV VILO Master Mix with ezDNase enzyme
Order now

SuperScript IV First Strand Synthesis System

SuperScript IV First-Strand Synthesis System

Flexibility in reaction conditions50–55°C10 min

cDNA synthesis kit
Order now

cDNA synthesis kit with ezDNase enzyme
Order now

uperScript IV First UniPrime One-Step RT-PCR system

SuperScript IV One-Step RT-PCR System

Fast and simple RT-PCR workflow

50–55°C

10 min

SuperScript IV UniPrime One-step RT-PCR system (colored)
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SuperScript IV UniPrime One-step RT-PCR system (dye-free)
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perScript IV Single Cell/Low Input cDNA PreAmp kit

SuperScript IV Single Cell/Low-Input cDNA PreAmp Kit

 

cDNA synthesis and preamplification from single cells or low quantities of RNA50°C10 minSuperScript IV Single Cell/Low-Input cDNA PreAmp Kit
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Product photo. Package and seven tubes of kit components.

SuperScript IV CellsDirect cDNA Synthesis Kit

Direct cDNA synthesis from mammalian cells50°C10 minSuperScript IV CellsDirect cDNA synthesis kit
Order now

Product photo. Package and five tubes of kit components.

SuperScript IV Template Switching RT Master Mix

Full length cDNA synthesis with enhanced template switching efficiency 30 min

SuperScript IV Template Switching RT Master Mix
Order now

Template Switching Reverse Transcription Reagents
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Comparison of SuperScript IV Reverse Transcriptase with other RTs

Stand-alone enzymes offer maximum flexibility in reaction setup. Table 2 compares the Superscript IV Reverse Transcriptase with other generations of reverse transcriptases.

Table 2. Comparison of standalone reverse transcriptases.

 M-MLV-Reverse Transcriptase
SuperScript II Reverse TranscriptaseSuperscript III Reverse Transcriptase
Superscript IV Reverse Transcriptase
Key attribute

Recombinant M-MLV RT for routine, non-demanding applications

Engineered M-MLV RT with reduced RNase H activity

Engineered M-MLV RT with reduced RNase H activity and improved thermal stability

Latest generation engineered RT for exceptional cDNA synthesis performance

Optimal reaction temperature37°C42°C50°C50–55°C
Reaction time50 min50 min30–50 min10 min
Sensitivity1 ng1 ng10 pg10 pg
Max cDNA lengthUp to 7 kbUp to 12.3 kbUp to 12.3 kb>12 kb
Ability to work with degraded or inhibitor-containing RNALowLowMediumHigh
Reduced RNase H activityNoYesYesYes


Performance of SuperScript IV Reverse Transcriptase

High sensitivity

Low Ct values. Ct values reduced by 8 cycles compared to other reverse transcriptases (Figure 2).

Figure 2. Sensitivity and variability in cDNA synthesis using degraded RNA samples. Degraded Arabidopsis total RNA (RIN: 1–3), in amounts of 1, 10, and 100 ng were used as input RNA in 20 μL reverse transcription reactions with SuperScript IV Reverse Transcriptase and random hexamers according to the product protocol. RTs from other vendors were used according to the manufacturers’ protocols. For each tested enzyme and each RNA input, RT reactions were performed in triplicates. From each reverse transcription reaction, 10% of the cDNA product was added to TaqMan assays for two targets, Gln synthetase and WRKY TF 70. Three qPCR reactions were performed for each reverse transcription reaction and the average Ct values for each RNA input were plotted (standard deviation from 9 Ct values for each input RNA).

Improved inhibitor tolerance

Compounds that have inhibitory effects on RTs are commonly found in RNA samples even after thorough purification. These compounds may interfere with cDNA synthesis, produce false RT-PCR and RT-qPCR results, and cause results to be misinterpreted. RT inhibitors include reagents used during RNA extraction, and co-purified contaminants arising from biological samples (Table 3). SuperScript IV Reverse Transcriptase shows significantly improved resistance to contaminating inhibitors when compared to previous generation SuperScript III Reverse Transcriptase and other commercially available RTs (Figure 3).

Table 3. Common cDNA synthesis inhibitors and their sources.

InhibitorSource
Ethanol/isopropanol, salts, phenol/chloroform, detergentsSample preparation
Hematin, bile saltsBlood, feces
Humic acid, polyphenols, polysaccharidesSoil, plants
Formalin, paraffinFFPE

Figure 3. Higher performance in cDNA synthesis in the presence of biological or sample prep inhibitors. A 0.5–10 kb RNA ladder was used in a 10 μL SuperScript IV Reverse Transcriptase reaction with oligo(dT)20 according to the product protocol. RTs from other vendors were used according to their respective protocols. Inhibitors were added to the RNA samples prior to primer annealing or addition of RT reaction mix. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using Invitrogen SYBR Gold Nucleic Acid Gel Stain. During electrophoresis, NaOH hydrolyzes all RNA, resulting in visualization of cDNA only.

Short reaction time

SuperScript IV Reverse Transcriptase has remarkable processivity and thereby generates long, full-length cDNA fragments within a short reaction time. Figure 4 demonstrates that SuperScript IV Reverse Transcriptase synthesized cDNAs of up to 9 kb in 10 minutes, while most other commercially available RTs were only able to synthesize cDNAs between 1.5–3 kb or less in the same duration.

Figure 4. Fast cDNA synthesis capability. The Invitrogen Millennium RNA Marker was used in a 10 μL reaction with SuperScript IV Reverse Transcriptase and oligo(dT) primer according to the product protocol. Other commercially available RTs were used according to manufacturers’ protocols, except for reaction times reduced to 10 minutes. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using SYBR Gold Nucleic Acid Gel Stain. During electrophoresis NaOH hydrolyzes all RNA, resulting in visualization of cDNA only.

High thermostability

When reverse transcription reactions are performed at low temperatures (less than 42°C), RNA secondary structures, especially GC-rich templates, may interfere with cDNA synthesis. SuperScript IV Reverse Transcriptase has high thermostability and can be used in reactions at 50°C or higher, which facilitates the successful transcription of highly structured RNA transcripts (Figure 5).

Figure 5. High thermostability of SuperScript IV Reverse Transcriptase. A 0.5–10 kb RNA ladder was used in a 10 μL SuperScript IV RT reaction with oligo(dT) according to the product protocol, with the exception that reaction temperature was varied between 50 and 65°C. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using SYBR Gold Nucleic Acid Gel Stain. During electrophoresis NaOH hydrolyzes all RNA, resulting in the visualization of cDNA only. cDNA bands were quantitated by TotalLab software for each reaction temperature. Percentage SuperScript IV RT activity was calculated by dividing values at each reaction temperature by values at 50°C.

Efficiency
High efficiency

Up to 100x higher cDNA yields with degraded RNA (Figure 1).

Figure 1. High efficiency with degraded RNA. RT-qPCR of degraded RNA (RIN 1–3) from human cells and plant tissues was performed with different brands of commercially available RTs and Applied Biosystems TaqMan assays. Delta Ct values (ΔCt = Ct – Ct SuperScript IV) show that SuperScript IV RT delivered up to 100x higher cDNA yields and has lower Ct values compared to SuperScript III and other commercial reverse transcriptases.

Sensitivity
High sensitivity

Low Ct values. Ct values reduced by 8 cycles compared to other reverse transcriptases (Figure 2).

Figure 2. Sensitivity and variability in cDNA synthesis using degraded RNA samples. Degraded Arabidopsis total RNA (RIN: 1–3), in amounts of 1, 10, and 100 ng were used as input RNA in 20 μL reverse transcription reactions with SuperScript IV Reverse Transcriptase and random hexamers according to the product protocol. RTs from other vendors were used according to the manufacturers’ protocols. For each tested enzyme and each RNA input, RT reactions were performed in triplicates. From each reverse transcription reaction, 10% of the cDNA product was added to TaqMan assays for two targets, Gln synthetase and WRKY TF 70. Three qPCR reactions were performed for each reverse transcription reaction and the average Ct values for each RNA input were plotted (standard deviation from 9 Ct values for each input RNA).

Inhibitor tolerance
Improved inhibitor tolerance

Compounds that have inhibitory effects on RTs are commonly found in RNA samples even after thorough purification. These compounds may interfere with cDNA synthesis, produce false RT-PCR and RT-qPCR results, and cause results to be misinterpreted. RT inhibitors include reagents used during RNA extraction, and co-purified contaminants arising from biological samples (Table 3). SuperScript IV Reverse Transcriptase shows significantly improved resistance to contaminating inhibitors when compared to previous generation SuperScript III Reverse Transcriptase and other commercially available RTs (Figure 3).

Table 3. Common cDNA synthesis inhibitors and their sources.

InhibitorSource
Ethanol/isopropanol, salts, phenol/chloroform, detergentsSample preparation
Hematin, bile saltsBlood, feces
Humic acid, polyphenols, polysaccharidesSoil, plants
Formalin, paraffinFFPE

Figure 3. Higher performance in cDNA synthesis in the presence of biological or sample prep inhibitors. A 0.5–10 kb RNA ladder was used in a 10 μL SuperScript IV Reverse Transcriptase reaction with oligo(dT)20 according to the product protocol. RTs from other vendors were used according to their respective protocols. Inhibitors were added to the RNA samples prior to primer annealing or addition of RT reaction mix. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using Invitrogen SYBR Gold Nucleic Acid Gel Stain. During electrophoresis, NaOH hydrolyzes all RNA, resulting in visualization of cDNA only.

Reaction time
Short reaction time

SuperScript IV Reverse Transcriptase has remarkable processivity and thereby generates long, full-length cDNA fragments within a short reaction time. Figure 4 demonstrates that SuperScript IV Reverse Transcriptase synthesized cDNAs of up to 9 kb in 10 minutes, while most other commercially available RTs were only able to synthesize cDNAs between 1.5–3 kb or less in the same duration.

Figure 4. Fast cDNA synthesis capability. The Invitrogen Millennium RNA Marker was used in a 10 μL reaction with SuperScript IV Reverse Transcriptase and oligo(dT) primer according to the product protocol. Other commercially available RTs were used according to manufacturers’ protocols, except for reaction times reduced to 10 minutes. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using SYBR Gold Nucleic Acid Gel Stain. During electrophoresis NaOH hydrolyzes all RNA, resulting in visualization of cDNA only.

Thermostability
High thermostability

When reverse transcription reactions are performed at low temperatures (less than 42°C), RNA secondary structures, especially GC-rich templates, may interfere with cDNA synthesis. SuperScript IV Reverse Transcriptase has high thermostability and can be used in reactions at 50°C or higher, which facilitates the successful transcription of highly structured RNA transcripts (Figure 5).

Figure 5. High thermostability of SuperScript IV Reverse Transcriptase. A 0.5–10 kb RNA ladder was used in a 10 μL SuperScript IV RT reaction with oligo(dT) according to the product protocol, with the exception that reaction temperature was varied between 50 and 65°C. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using SYBR Gold Nucleic Acid Gel Stain. During electrophoresis NaOH hydrolyzes all RNA, resulting in the visualization of cDNA only. cDNA bands were quantitated by TotalLab software for each reaction temperature. Percentage SuperScript IV RT activity was calculated by dividing values at each reaction temperature by values at 50°C.

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Resources for SuperScript IV Reverse Transcriptase

Interactive selection tool: Find the right reverse transcriptase for your experiments.

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    Watch this video on the advantages of SuperScript IV Reverse Transcriptase.


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      Understand how SuperScript IV Reverse Transcriptase consistently delivers low Ct values in qPCR reactions.

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          Learn how the SuperScript IV UniPrime One-Step RT-PCR System makes it easy to set up reactions while bringing superior results.

          Manuals

          Protocols

          Technical articles


          SuperScript IV Reverse Transcriptase FAQs

          Find tips, troubleshooting help, and resources for common questions about SuperScript Reverse Transcriptases. Can’t find your question? Search the FAQ database

          What are the key differences between Invitrogen SuperScript III Reverse Transcriptase and Invitrogen SuperScript IV Reverse Transcriptase?

          The SuperScript IV enzyme has been engineered for higher thermostability, processivity, and cDNA yields. It performs better in the presence of inhibitors, and the reaction buffer has also been optimized for robust cDNA synthesis from a wide range of samples.

          Can I get a comparable cDNA yield and length using the SuperScript IV RT 10-minute protocol as when using a 50-minute protocol for SuperScript III RT?

          When compared with SuperScript III RT (and other manufacturers’ RTs) in a synthesis reaction for a 9 kb cDNA, SuperScript IV RT performed successful synthesis in just 10 minutes and did so with comparable (or improved) yield.

          Can SuperScript IV RT be used at higher reaction temperatures to reverse transcribe RNAs with secondary structures?

          SuperScript IV RT sustains 100% activity at up to 56.4°C and 70% activity at up to 65°C, while wild type MMLV RT or MMLV RNase H– RT enzymes usually display very low or no activity above 45°C. SuperScript IV RT’s ability to function at higher temperatures enables the reverse transcription of RNA targets with structural complexities.


          SuperScript IV Reverse Transcriptases: Citations

          SuperScript IV reverse transcriptases are highly cited in the several peer reviewed research publications. In three years, between 2022 and 2024, it has been cited over in 10K publications.

          Cancer
          UseReference

          Detect cancer biomarkers from extracellular vesicles by ddPCR (digital droplet).

          		Lee KY, Beatson EL, Knechel MA et al. (2024). Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3. J Cancer 15(3):615–622.
          doi: 10.7150/jca.90836. PMID: 38213719.

          cDNA synthesis for preparation of a Poly(A)-seq library and also for nested RT-PCR to study changes in UTR length.

          		Gabel AM, Belleville AE, Thomas JD et al. (2024). Multiplexed screening reveals how cancer-specific alternative polyadenylation shapes tumor growth in vivo.Nat Commun 15:959.
          doi: 10.1038/s41467-024-44931-x. PMID: 38302465

          Synthesize cDNA from FFPE samples for ddPCR.

          		Look T, Puca E, Bühler M et al. (2023). Targeted delivery of tumor necrosis factor in combination with CCNU induces a T cell-dependent regression of glioblastoma. Sci Transl Med 15(697).
          doi:10.1126/scitranslmed.adf2281 PMID: 37224228
          Viral and infectious diseases
          UseReference

          Reverse transcribe viral RNA to cDNA prior to circularization, RCA, and nanopore sequencing.

          		Misu M, Yoshikawa T, Sugimoto S (2023). Rapid whole genome sequencing methods for RNA viruses. Front Microbiol 23: 14.
          doi: 10.3389/fmicb.2023.1137086. PMID: 36910229

          Reverse transcribe avian viral RNA prior to amplification and library prep.

          		Narvaez SA, Harrell TL, Oluwayinka (2023). Optimizing the Conditions for Whole-Genome Sequencing of Avian Reoviruses. Viruses 15(9):1938.
          doi: 10.3390/v15091938. PMID: 37766345

          Substituted SuperScript IV Reverse Transcriptase for RT in Illumina kit for library prep and had improved depth of sequencing.

          		Soufi EG, Jorio DL, Gerber Z et al (2024). Highly efficient and sensitive membrane-based concentration process allows quantification, surveillance, and sequencing of viruses in large volumes of wastewater. Water Res 249.
          doi: 10.1016/j.watres.2023.120959. PMID: 38070350

          Measure concatemeric cDNA formation upon phage infection. This ccDNA encodes for a toxic protein.

          		Wilkinson ME, Li D, Gao A et al. (2024). Phage-triggeredreverse transcription assembles a toxic repetitive gene from a noncoding RNA. Science 386 (6717).
          doi:10.1126/science.adq3977 PMID: 39208082

          Synthesize cDNA from HIV RNA from patients. The amplified DNA was analyzed by gel elecrophoresis and NGS. It was also cloned and sequenced by Sanger sequencing.

          		Esmaeilzadeh E, Etemad B, Lavine CL et al. (2023). Autologous neutralizing antibodies increase with early antiretroviral therapy and shape HIV rebound after treatment interruption. Sci Transl Med 5(695).
          doi:10.1126/scitranslmed.abq4490
          Immunology
          UseReference

          Characterize monoclonal antibodies from B-cells. Developed a switching mechanism at the 5′ ends of the RNA transcript (SMART)-based method for amplifying IgV genes from single RM B cells to capture Ig heavy and light chain pairs.

          		Weinfurter JT, Bennett SN, Reynolds MR (2024). A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells.J Immunol Methods 525:113602.
          doi: 10.1016/j.jim.2023.113602 PMID: 38103783

          Synthesize cDNA for RT-qPCR to study changes to gene expression as a result of CRISPR knock out of transcription factor Mef2d.

          		Szeto ACH, Clark PA, Ferreira ACF et al. (2024). Mef2d potentiates type-2 immune responses and allergic lung inflammation. Science 384(6703).
          doi:10.1126/science.adl0370 PMID: 38935708

          Synthesize cDNA to measure the expression of Epstein bar virus transcripts.

          		Zhang TT, Cheng RY, Ott AR, et al (2024). BCR signaling is required for posttransplant lymphoproliferative disease in immunodeficient mice receiving human B cells. Sci Transl Med 16(742).
          doi:10.1126/scitranslmed.adh8846 PMID: 38598616
          Synthesize cDNA from human B cells. cDNA was amplified and sequenced.Grigoryan L, Feng Y, Bellusci L et al. (2024). AS03 adjuvant enhances the magnitude, persistence, and clonal breadth of memory B cell responses to a plant-based COVID-19 vaccine in humans. Sci Immunol 9(94).
          doi:10.1126/sciimmunol.adi8039 PMID: 38579013

          cDNA synthesis for RT-qPCR. Patient samples were used.

          		Morot J, Del Duca E, Chastagner M et al. (2023). Hyperactivation of the JAK2/STAT5 signaling pathway and evaluation of baricitinib treatment among patients with eosinophilic cellulitis. JAMA Dermatol 159(8):820–829.
          doi: 10.1001/jamadermatol.2023.1651 PMID: 37342057

          Synthesize cDNA from human B cells. cDNA was amplified and sequenced.

          		Dejoux A, Zhu Q, Ganneau C, et al. (2024). Rocuronium-specific antibodies drive perioperative anaphylaxis but can also function as reversal agents in preclinical models. Sci Transl Med 16(764).
          doi:10.1126/scitranslmed.ado4463 PMID: 39259810
          Ecology and Evolutionary Biology
          UseReference

          Study mRNA expression profiles in tissue and identify a unique pathway in a shark species.

          		Cutler CP, Omoregie E, Ojo T. UT-1 Transporter Expression in the Spiny Dogfish (Squalus acanthias): UT-1 Protein Shows a Different Localization in Comparison to That of Other Sharks. Biomolecules 14(9):1151.
          doi: 10.3390/biom14091151. PMID: 39334917

          Used SuperScript IV Reverse Transcriptase with low input sample difficult sample (RNA from insect antennae).

          		Johny J, Nihad M, Alharbi HA et al. (2024). Silencing sensory neuron membrane protein RferSNMPu1 impairs pheromone detection in the invasive Asian Palm Weevil.Sci Rep 14(1):16541.
          doi: 10.1038/s41598-024-67309-x. PMID: 39019908

          Synthesize cDNA from RNA isolated from yeast cells to measure gene expression.

          		Montrose K, Lac DT, Burnetti AJ et al.(2024). Proteostatic tuning underpins the evolution of novel multicellular traits. Sci Adv 10(10).
          doi:10.1126/sciadv.adn2706 PMID: 38457507
          Other areas
          UseReference

          Convert RNA to cDNA prior to a qPCR reaction to measure the relative expression levels of target genes in wound healing assays.

          		Rapp J, Ness J, Wolf J (2024). 2D and 3D in vitro angiogenesis assays highlight different aspects of angiogenesis. Biochim Biophys Acta Mol Basis Dis 1870(3).
          doi: 10.1016/j.bbadis PMID: 38244944

          Synthesize cDNA for the study of RNA splicing. Products were analyzed by agarose gel electrophoresis, qPCR, and Sanger sequencing.

          		Žedaveinytė R, Meers C, Le HC et al. (2024). Antagonistic conflict between transposon-encoded introns and guide RNAs. Science 385(6705).
          doi:10.1126/science.adm8189 PMID: 38991068

          Synthesize cDNA from CRISPR modifed cells for RNA-seq.

          		Dudnyk K, Cai D, Shi C, Xu J et al. (2024). Sequence basis of transcription initiation in the human genome. Science 384(6694).
          doi:10.1126/science.adj0116 PMID: 38662817
          Cancer
          Cancer
          UseReference

          Detect cancer biomarkers from extracellular vesicles by ddPCR (digital droplet).

          		Lee KY, Beatson EL, Knechel MA et al. (2024). Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3. J Cancer 15(3):615–622.
          doi: 10.7150/jca.90836. PMID: 38213719.

          cDNA synthesis for preparation of a Poly(A)-seq library and also for nested RT-PCR to study changes in UTR length.

          		Gabel AM, Belleville AE, Thomas JD et al. (2024). Multiplexed screening reveals how cancer-specific alternative polyadenylation shapes tumor growth in vivo.Nat Commun 15:959.
          doi: 10.1038/s41467-024-44931-x. PMID: 38302465

          Synthesize cDNA from FFPE samples for ddPCR.

          		Look T, Puca E, Bühler M et al. (2023). Targeted delivery of tumor necrosis factor in combination with CCNU induces a T cell-dependent regression of glioblastoma. Sci Transl Med 15(697).
          doi:10.1126/scitranslmed.adf2281 PMID: 37224228
          Viral and infectious disease
          Viral and infectious diseases
          UseReference

          Reverse transcribe viral RNA to cDNA prior to circularization, RCA, and nanopore sequencing.

          		Misu M, Yoshikawa T, Sugimoto S (2023). Rapid whole genome sequencing methods for RNA viruses. Front Microbiol 23: 14.
          doi: 10.3389/fmicb.2023.1137086. PMID: 36910229

          Reverse transcribe avian viral RNA prior to amplification and library prep.

          		Narvaez SA, Harrell TL, Oluwayinka (2023). Optimizing the Conditions for Whole-Genome Sequencing of Avian Reoviruses. Viruses 15(9):1938.
          doi: 10.3390/v15091938. PMID: 37766345

          Substituted SuperScript IV Reverse Transcriptase for RT in Illumina kit for library prep and had improved depth of sequencing.

          		Soufi EG, Jorio DL, Gerber Z et al (2024). Highly efficient and sensitive membrane-based concentration process allows quantification, surveillance, and sequencing of viruses in large volumes of wastewater. Water Res 249.
          doi: 10.1016/j.watres.2023.120959. PMID: 38070350

          Measure concatemeric cDNA formation upon phage infection. This ccDNA encodes for a toxic protein.

          		Wilkinson ME, Li D, Gao A et al. (2024). Phage-triggeredreverse transcription assembles a toxic repetitive gene from a noncoding RNA. Science 386 (6717).
          doi:10.1126/science.adq3977 PMID: 39208082

          Synthesize cDNA from HIV RNA from patients. The amplified DNA was analyzed by gel elecrophoresis and NGS. It was also cloned and sequenced by Sanger sequencing.

          		Esmaeilzadeh E, Etemad B, Lavine CL et al. (2023). Autologous neutralizing antibodies increase with early antiretroviral therapy and shape HIV rebound after treatment interruption. Sci Transl Med 5(695).
          doi:10.1126/scitranslmed.abq4490
          Immunology
          Immunology
          UseReference

          Characterize monoclonal antibodies from B-cells. Developed a switching mechanism at the 5′ ends of the RNA transcript (SMART)-based method for amplifying IgV genes from single RM B cells to capture Ig heavy and light chain pairs.

          		Weinfurter JT, Bennett SN, Reynolds MR (2024). A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells.J Immunol Methods 525:113602.
          doi: 10.1016/j.jim.2023.113602 PMID: 38103783

          Synthesize cDNA for RT-qPCR to study changes to gene expression as a result of CRISPR knock out of transcription factor Mef2d.

          		Szeto ACH, Clark PA, Ferreira ACF et al. (2024). Mef2d potentiates type-2 immune responses and allergic lung inflammation. Science 384(6703).
          doi:10.1126/science.adl0370 PMID: 38935708

          Synthesize cDNA to measure the expression of Epstein bar virus transcripts.

          		Zhang TT, Cheng RY, Ott AR, et al (2024). BCR signaling is required for posttransplant lymphoproliferative disease in immunodeficient mice receiving human B cells. Sci Transl Med 16(742).
          doi:10.1126/scitranslmed.adh8846 PMID: 38598616
          Synthesize cDNA from human B cells. cDNA was amplified and sequenced.Grigoryan L, Feng Y, Bellusci L et al. (2024). AS03 adjuvant enhances the magnitude, persistence, and clonal breadth of memory B cell responses to a plant-based COVID-19 vaccine in humans. Sci Immunol 9(94).
          doi:10.1126/sciimmunol.adi8039 PMID: 38579013

          cDNA synthesis for RT-qPCR. Patient samples were used.

          		Morot J, Del Duca E, Chastagner M et al. (2023). Hyperactivation of the JAK2/STAT5 signaling pathway and evaluation of baricitinib treatment among patients with eosinophilic cellulitis. JAMA Dermatol 159(8):820–829.
          doi: 10.1001/jamadermatol.2023.1651 PMID: 37342057

          Synthesize cDNA from human B cells. cDNA was amplified and sequenced.

          		Dejoux A, Zhu Q, Ganneau C, et al. (2024). Rocuronium-specific antibodies drive perioperative anaphylaxis but can also function as reversal agents in preclinical models. Sci Transl Med 16(764).
          doi:10.1126/scitranslmed.ado4463 PMID: 39259810
          Ecology and evolutionary biology
          Ecology and Evolutionary Biology
          UseReference

          Study mRNA expression profiles in tissue and identify a unique pathway in a shark species.

          		Cutler CP, Omoregie E, Ojo T. UT-1 Transporter Expression in the Spiny Dogfish (Squalus acanthias): UT-1 Protein Shows a Different Localization in Comparison to That of Other Sharks. Biomolecules 14(9):1151.
          doi: 10.3390/biom14091151. PMID: 39334917

          Used SuperScript IV Reverse Transcriptase with low input sample difficult sample (RNA from insect antennae).

          		Johny J, Nihad M, Alharbi HA et al. (2024). Silencing sensory neuron membrane protein RferSNMPu1 impairs pheromone detection in the invasive Asian Palm Weevil.Sci Rep 14(1):16541.
          doi: 10.1038/s41598-024-67309-x. PMID: 39019908

          Synthesize cDNA from RNA isolated from yeast cells to measure gene expression.

          		Montrose K, Lac DT, Burnetti AJ et al.(2024). Proteostatic tuning underpins the evolution of novel multicellular traits. Sci Adv 10(10).
          doi:10.1126/sciadv.adn2706 PMID: 38457507
          Miscellaneous
          Other areas
          UseReference

          Convert RNA to cDNA prior to a qPCR reaction to measure the relative expression levels of target genes in wound healing assays.

          		Rapp J, Ness J, Wolf J (2024). 2D and 3D in vitro angiogenesis assays highlight different aspects of angiogenesis. Biochim Biophys Acta Mol Basis Dis 1870(3).
          doi: 10.1016/j.bbadis PMID: 38244944

          Synthesize cDNA for the study of RNA splicing. Products were analyzed by agarose gel electrophoresis, qPCR, and Sanger sequencing.

          		Žedaveinytė R, Meers C, Le HC et al. (2024). Antagonistic conflict between transposon-encoded introns and guide RNAs. Science 385(6705).
          doi:10.1126/science.adm8189 PMID: 38991068

          Synthesize cDNA from CRISPR modifed cells for RNA-seq.

          		Dudnyk K, Cai D, Shi C, Xu J et al. (2024). Sequence basis of transcription initiation in the human genome. Science 384(6694).
          doi:10.1126/science.adj0116 PMID: 38662817

          Resources

          Reverse transcriptase selection tool
          Find the right products to fit your cDNA synthesis needs.

          Reverse transcriptase webinars
          Stay up-to-date on current industry trends in cDNA synthesis applications with on-demand webinars.

          Reverse transcriptase literature
          Access white papers and technical and application notes for insights on cutting edge reverse transcription technology.

          Reverse transcriptase videos
          Watch instructional videos on troubleshooting tips, how-to videos, and explainers related to cDNA synthesis.

          Reverse transcriptase education
          Review educational resources on topics including reverse transcription basics, cDNA synthesis, enzyme selection, troubleshooting tips, and cDNA applications in molecular biology.

          Support

          PCR and cDNA Synthesis Support Center
          Find support for every step of your cDNA synthesis experiments.

          Tm calculator
          Handy tool to calculate Tm (melting temperature) of primers and annealing temperatures.

          Contact us
          Get in touch with our technical application scientists for questions regarding reverse transcription enzymes, master mixes, and cDNA synthesis kits.

          Mobile and desktop applications
          Access content and helpful tools through SmartIST and Cloning Bench mobile apps.

          OEM and custom solutions
          Source reverse transcriptase and other molecular biology enzymes, PCR reagents, and plastics customized to your needs.

          For Research Use Only. Not for use in diagnostic procedures.

          Stylesheet for Classic Wide Template adjustments