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All Invitrogen precast protein gels are high-quality polyacrylamide gels designed to deliver excellent performance, reliability, and reproducibility. Our protein gels are offered in four different chemistries. Whether your protein is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native conditions or by isoelectric point—we have a solution for your application.
Learn about WedgeWell protein gels
Four gel chemistries for tailored SDS-PAGE separation
Exceptional protein gel electrophoresis is the cornerstone of a successful western blotting experiment. Invitrogen precast protein gels are offered in four different chemistries, allowing you to tailor the separation to your target protein's molecular weight. This helps obtain sharp, well-resolved bands, and results in efficient transfer and improved detection of your protein.
 Bis-Tris Gels |  Tris-Glycine Gels |  Tris-Acetate Gels |  Tricine Gels | |
| Available precast gels | NuPAGE Bis-Tris Gels, Bolt Bis-Tris Plus Gels | Novex Tris-Glycine | NuPAGE Tris-Acetate | Novex Tricine |
| Use for | Neutral pH environment helps prevent protein degradation Faster run times Dilute samples, low abundance proteins | Traditional Laemmli-style gel electrophoresis | Improved resolution of high molecular weight proteins | Improved resolution of low molecular weight proteins |
| Protein sample type | Broad range MW (6–400 kDa) | Broad range MW (6–400 kDa) | High range MW (40–500 kDa) | Low range MW (2.5–40 kDa) |
| WedgeWell format gels | Midi and mini (load up to 100 and 60 µL, respectively) | Midi and mini (load up to 100 and 60 µL, respectively) | Midi and mini (load up to 100 and 60 µL, respectively) | - |
| Average run time | 22–45 min Bolt gels 35–50 min NuPAGE | 90 min | 60 min | 90 min |
| Shelf life* | Up to 16 months at room temperature | Up to 12 months | Up to 8 months | 1–2 months |
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High loading capacity meets high sample throughput
WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to 100 µL of sample to be loaded in the well. The WedgeWell design was introduced in 2012 to increase the sample loading capacity of Invitrogen precast mini gels.
Figure 1.Comparison of a Mini WedgeWell well and traditional Mini gel well shape.
More than a thousand peer-reviewed articles demonstrate how WedgeWell format gels help produce publication-quality results and enable the study of low abundance proteins, reducing the need to enrich dilute samples before loading them onto the gel.
Midi gels with WedgeWell format wells allow the separation of up to 26 protein samples in the same run without compromising on sample loading volume.
Key benefits of WedgeWell format gels:
- Results you can trust—rigorously tested to help ensure reliable, reproducible performance, lot after lot
- Easy sample loading—larger well openings make loading a breeze even with standard pipette tips, and reduce sample spill-over and cross well contamination
- Increased sample loading capacity—easy detection of dilute samples and low abundance proteins
- Reduced cost—lower cost per sample and reduced running buffer required per sample (7.3 mL of running buffer saved per sample, 140 mL saved for 20 samples)*
- Faster run times—up to 10 min faster than traditional gels
Available in three different gel chemistries:
All Invitrogen protein gels meet the highest standards of performance, reliability, and consistency.
See our Protein Gel Performance Guarantee
*Based on comparison between our most popular mini and midi well format options (12 and 20 well, respectively)
Recommended sample loading volumes for mini and midi gels
| Well size | Maximum loading volume (µL) | ||
| WedgeWell format midi gels | Standard midi gels | ||
|---|---|---|---|
| 12 + 2 (small wells) | 100 + 35 | 50 + 15 | |
| 20 | 60 | 30 | |
| 26 | 40 | 20 | |
| WedgeWell format mini gels | Standard mini gels | ||
| 10 | 60 | 25 | |
| 12 | 45 | 20 | |
| 15 | 35 | 15 | |
| 17 | 30 | 15 | |
IEF and specialized gels
For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.
For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.
270x195.png) NativePAGE Bis-Tris Gels |  Isoelectric Focusing Gels |  Novex Zymogram Gels |  E-PAGE Precast Gels | |
| Gel chemistry | Bis-Tris | pH 3–7, pH 3–10 | Tris-glycine with gelatin | 8% or 6% |
| Protein sample type | 15–10,000 kDa | pI 3.5–8.5 | 10–220 kDa | 10–200 kDa |
| Product benefits | Separation of native proteins, neutral pH for high sensitivity applications | Separation of proteins based on isoelectric point | Separation of proteins based on size and activity | High-throughput gel system, that eliminates the need for buffers |
Handcast System
 SureCast Handcast System |  Empty Gel Cassettes |  Bolt Empty Cassettes | |
| Cassette type | Reusable, glass | Single use, pre-sealed | Single use, pre-sealed |
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| Gel dimensions | Mini: 8x8 cm (1 mm thick) | Mini: 8x8 cm (1 or 1.5 mm thick) Midi: 8x13 cm (1 mm thick) | Mini: 8x8 cm (1 mm thick) |
| Gel tank | Mini Gel Tank, Xcell SureLock Mini-Cell | Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only) | Mini Gel Tank, Xcell SureLock Mini-Cell |
| Reagents | SureCast reagents, other popular polyacrylamide reagents | SureCast reagents, other popular polyacrylamide reagents | SureCast reagents, other popular polyacrylamide reagents |
| Combos available | 1-, 2-, 10-, 12-, and 15-wells | 1-, 2-, 5-, 10-, 12-, 15-, and 2D-wells | 10- or 12-wells (high capacity wells) |
How-to videos
How to choose the right protein gel for your research application
This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications, and how to introduce the right gel chemistry in your research workflow.
How to separate proteins using an Invitrogen precast SDS-PAGE gel
This video will show you how to separate proteins by using Invitrogen Mini Gel Tank and Invitrogen precast SDS-PAGE gels.
Find an excellent mini or midi gel for your protein electrophoresis experiment.
Find an exceptional Invitrogen mini or midi gel to replace your current precast gel from another supplier.
Download the Protein Gel Guide for a printable gel migration chart.
Still unsure which precast gel works best for your research needs? Download the Gel Selection Chart
References
- Libardo, M.D.J.; Durr, E.; Hernandez, L.D. A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable Haemophilus influenzae.Methods Protoc.2023,6, 42. https://doi.org/10.3390/mps6020042
- Horita, H., Law, A., Middleton, K. Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins. J. Vis. Exp. (131), e56912, doi:10.3791/56912 (2018).
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For Research Use Only. Not for use in diagnostic procedures.