DNAzol™ BD Reagent, for isolation of genomic DNA from whole blood

DNAzol™ BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood inRead more

Catalog Number	Quantity
10974020	100 mL

Catalog number 10974020

Price (USD)

514.00

Each

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Quantity:

100 mL

Price (USD)

514.00

Each

Add to cart

DNAzol™ BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes.

• Specifically formulated for extraction of genomic DNA from whole blood
• Optimized for the rapid Isolation of genomic DNA

Efficient Isolation of Genomic DNA from Whole Blood
DNAzol™ BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol™ BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. 1 ml of DNAzol™ Reagent can be used to isolate genomic DNA from 0.5 ml of whole blood to yield 10-20 μg DNA. In addition to the isolation of genomic DNA, DNAzol™ BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum.

Rapid Isolation of Genomic DNA
The DNAzol™ Reagent combines reliability, efficiency and simplicity in its isolation protocol. In the procedure, blood samples are mixed with DNAzol™ Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is then washed successively with DNAzol™ Reagent followed by ethanol, and then solubilized. The entire procedure can be completed in about 30 min.

Isolated DNA can be Used for a Number of Downstream Applications:
Genomic DNA extracted using the DNAzol™ procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product TypeGenomic DNA

For Use With (Application)PCR, Southern Blotting, Sequencing, Cloning

High-throughput CompatibilityNot High-throughput Compatible (Manual)

Product LineDNAzol™

Product TypeBD Reagent

Quantity100 mL

Sample TypeBlood

Shipping ConditionRoom Temperature

TargetGenomic DNA

Test Time30 min.

Isolation TechnologyOrganic Extraction

Unit SizeEach

Contents & Storage

Store at room temperature.

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Frequently asked questions (FAQs)

I've isolated red DNA from my blood sample using DNAzol BD Reagent. Why is this and is it okay to use?

The color is due to cells lysing before the DNAzol Reagent is added. The color is caused by hemoglobin. This contaminant will cause problems during PCR, and must be removed before the ethanol wash step to prevent them. The following are possible reasons for the contamination:

- Inadequate or ineffective anticoagulants can result in clot formation; typically, this will give DNA a speckled appearance. Remove them by centrifugation before precipitating the DNA.
- The walls of the tube were not adequately washed with the DNAzol BD wash solution. Trace amounts of protein on the wall of the tube can cause contamination if this residue is dislodged during the ethanol wash steps.

Will DNAzol Reagent isolate only genomic DNA or will plasmid DNA also be isolated? How about mitochondrial DNA?

The DNA isolated is actually total DNA, so plasmid DNA will be isolated along with genomic DNA. The mitochondrial genome is similar to a plasmid and can be isolated using DNAzol Reagent. The 1 minute room temperature incubation in ethanol before centrifugation should be extended to 5-10 minutes for maximum recovery.

At what temperatures can DNAzol reagent be stored and still be okay to use? Are there possible stopping/storage points in the protocol?

We recommend storing DNAzol Reagent at room temperature. The DNAzol lysate (homogenate) can be stored 1 month at 15-30 degrees C; after 10 months at 4 degrees C or -20 degrees C, the DNAzol lysate (homogenate) has yielded high molecular weight genomic DNA, which can be completely digested with restriction enzymes and works well in PCR. During washes, DNA can be stored in 95% EtOH for at least one week at 15 degrees C to 30 degrees C or for three months at approximately 4 degrees C. DNA can be stored in DNAzol Reagent for one month at room temperature or 10 months at 4 degrees C.

What kit should I use for blood genomic DNA isolation?

Please see the following web page (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/blood-dna-extraction.html) for a comparison of kits that can be used to isolate genomic DNA from blood.

After DNAzol reagent extraction, my OD 260/280 ratio is low. Does this mean I have poor-quality DNA?

Consider the following if you have a low 260/280 ratio:

The correct amount of DNAzol reagent may not have been used. If DNAzol reagent was added to a cell pellet, make sure that the volume of reagent is 20 times that of the cell pellet.

There may have been a problem in pipetting away the viscous supernatant from the DNA pellet, leading to contamination with protein. The DNA may be used with DNAzol reagent again or extracted with phenol to remove the protein.

In some samples dissolved in water, the ratio may be low due to the acidity of the water or the low ion content in the water. The ratios may go up if the sample is dissolved in TE and the spec is zeroed with TE (or 1 to 3 mM Na2PO4, pH ~8.0). [See BioTechniques 22:474-6, 478-81 (1997).]. The molar extinction coefficient of the nucleotides is given at neutral pH, suggesting that the absorbance at 260 nm would be highest at neutral pH. Of course, DNA is not stable under acidic conditions so degradation may occur if the DNA is left in this condition for too long.