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Waymouth's Medium
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Waymouth's Medium
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Citations & References (2)
Gibco™

Waymouth's Medium

Waymouth's is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, andRead more
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Catalog NumberQuantity
11220035Promo Image500 mL
Catalog number 11220035
Price (USD)
55.25
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Waymouth's is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, and growth of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Waymouth's medium is also used for the culture of whole organs and a broad range of cell types, such as carcinoma cell lines.

This Waymouth's medium is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell LineL929, carcinomas
Cell TypeWhole Organs
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeWaymouth's Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
11220 - Waymouth's
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Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Waymouth's Medium FAQs

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Certificates

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Lot #Certificate TypeDateCatalog Number(s)
3207749Certificate of AnalysisJul 23, 202511220035
2584461Certificate of AnalysisJan 29, 202511220035
3079793Certificate of AnalysisJan 07, 202511220035
3002206Certificate of AnalysisSep 13, 202411220035
2998892Certificate of AnalysisAug 08, 202411220035
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SDS

Scientific Resources

Citations & References (2)

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Citations & References
Abstract
Ets-1 positively regulates Fas ligand transcription via cooperative interactions with Sp1.
Authors:Kavurma MM, Bobryshev Y, Khachigian LM,
Journal:J Biol Chem
PubMed ID:11970950
'The FasL/Fas system has been implicated in smooth muscle cell apoptosis and atherosclerotic plaque instability, a process that can lead to plaque rupture, precipitating myocardial infarction and sudden death. The transcriptional mechanisms regulating FasL gene expression in vascular smooth muscle cells are poorly understood. We recently described a novel mechanism ... More
Intracellular third loop domain of angiotensin II type-2 receptor. Role in mediating signal transduction and cellular function.
Authors: Hayashida W; Horiuchi M; Dzau V J;
Journal:J Biol Chem
PubMed ID:8703004
'The present study tests the hypothesis that the unique intracellular third loop domain of angiotensin II type-2 (AT2) receptor is essential for the subsequent intracellular signaling and plays an important role in mediating receptor function. Synthetic intracellular third loop peptide of the AT2 receptor (AT2-3LP, 22 amino acids) and control ... More
2 total citations

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