1x1 image pixel for data collection
DMEM, high glucose, pyruvate
Save up to 29% on your order of cell culture products. View promotion details >
DMEM, high glucose, pyruvate
Gibco™

DMEM, high glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
Promo ImagePromotion
Promo code:RCELL25
Save up to 29% across the cell culture workflow - research begins with cells
From now until Dec 31, get up to 29% off media, sera, buffers, reagents and other cell culture products to advance your researchLearn More
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
11995065Promo Image500 mL
11995073Promo Image10 x 500 mL
11995040Promo Image1000 mL
11995081Promo Image6 x 1000 mL
11995115Promo Image5 L
11995123Promo Image10 L
Catalog number 11995065
Price (USD)
34.65
Online Exclusive
37.43
Save 2.78 (7%)
Each
In stock
Add to cart
Quantity:
500 mL
Recurring order eligible. Learn more »
Customize this product
Price (USD)
34.65
Online Exclusive
37.43
Save 2.78 (7%)
Each
Add to cart
Ask our AI about this Product

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red, Sodium pyruvate

Without: HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionAmbient
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

What is the difference between DMEM, high glucose, pyruvate Cat. No. 41966029 and Cat. No. 11995065?

Cat. No. 41966029 is manufactured in the Paisley, Scotland facility, while Cat. No. 11995065 is dual manufactured in Grand Island, New York and Paisley, Scotland. Although the formulation is said to be equivalent for molarities, the components may differ (e.g., it could be that one used L-Cystine 2HCl the other L-Cystine). This should not affect performance. Both facilities are FDA-registered and ISO 13485 certified.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3157163Certificate of AnalysisJul 09, 202511995065, 11995123, 11995115, 11995040
3140621Certificate of AnalysisJun 11, 202511995065, 11995123, 11995115, 11995040
3144488Certificate of AnalysisJun 05, 202511995065, 11995123, 11995115, 11995040
3142738Certificate of AnalysisMay 15, 202511995065, 11995123, 11995115, 11995040
3142744Certificate of AnalysisMay 11, 202511995065, 11995123, 11995115, 11995040
5 results displayed, search above for a specific certificate

Safety Data Sheets

Citations & References (8)

Citations & References
Abstract
Astrocytes in culture produce anandamide and other acylethanolamides.
Authors: Walter Lisa; Franklin Allyn; Witting Anke; Moller Thomas; Stella Nephi;
Journal:J Biol Chem
PubMed ID:11916961
'Anandamide (arachidonylethanolamide) is an endocannabinoid that belongs to the acylethanolamide lipid family. It is produced by neurons in a calcium-dependent manner and acts through cannabinoid CB1 receptors. Other members of the acylethanolamide lipid family are also produced by neurons and act through G-protein-coupled receptors: homo-gamma-linolenylethanolamide (HEA) and docosatetraenylethanolamide (DEA) act ... More
PRiMA: the membrane anchor of acetylcholinesterase in the brain.
Authors: Perrier Anselme L; Massoulie Jean; Krejci Eric;
Journal:Neuron
PubMed ID:11804574
'As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. We have previously shown that collagen Q (ColQ) anchors AChE at the neuromuscular junction. We have now cloned the gene PRiMA (proline-rich membrane anchor) encoding the AChE anchor ... More
Plaque production by the polyoma virus.
Authors:DULBECCO R, FREEMAN G,
Journal:Virology
PubMed ID:13669362
Describes the original formulation of DMEM (Dulbecco’s Modified Eagle Medium), used for plaque production by the polyoma virus.
Interaction between GRIP and Liprin-alpha/SYD2 Is Required for AMPA Receptor Targeting.
Authors: Wyszynski Michael; Kim Eunjoon; Dunah Anthone W; Passafaro Maria; Valtschanoff Juli G; Serra-Pagès Carles; Streuli Michel; Weinberg Richard J; Sheng Morgan;
Journal:Neuron
PubMed ID:11931740
Interaction with the multi-PDZ protein GRIP is required for the synaptic targeting of AMPA receptors, but the underlying mechanism is unknown. We show that GRIP binds to the liprin-alpha/SYD2 family of proteins that interact with LAR receptor protein tyrosine phosphatases (LAR-RPTPs) and that are implicated in presynaptic development. In neurons, ... More
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Authors: Esumi Hiroyasu; Izuishi Kunihiko; Kato Kazuyoshi; Hashimoto Koichi; Kurashima Yukiko; Kishimoto Atsuhiro; Ogura Tsutomu; Ozawa Takayuki;
Journal:J Biol Chem
PubMed ID:12091379
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia; ... More
8 total citations

Other products to consider



Share catalog number, name or link

1x1 image pixel for data collection