Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System
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Invitrogen™

Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast VirusRead more
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Catalog NumberNo. of Reactions
11731023500 Reactions
11731015100 Reactions
Catalog number 11731023
Price (USD)
5,230.00
Each
-
Add to cart
No. of Reactions:
500 Reactions
Recurring order eligible. Learn more »
Price (USD)
5,230.00
Each
Add to cart
Ask our AI about this Product
Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast Virus 1-Step Master Mix, we’ve improved on the best features of the Platinum Quantitative RT-PCR ThermoScript One-Step System and added additional convenience and performance to your 1-step gene expression analysis and RNA virus detection workflows.

Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

This kit provides:
• Platinum™ Taq DNA Polymerase
• ThermoScript RT polymerase
• A vial of 50 mM MgSO4

For Research Use Only. Not for use in diagnostic procedures.
Specifications
No. of Reactions500 Reactions
PolymeraseTaq DNA Polymerase
Product LinePlatinum™, ThermoScript™
Product TypeRT-PCR One-Step System
Quantity500 reactions
Sample TypeRNA
Shipping ConditionDry Ice
Sufficient For500 Reactions
Detection MethodPrimer-probe
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3147150Certificate of AnalysisFeb 11, 202511731023
3049018Certificate of AnalysisNov 04, 202411731015, 11731-015
3048079Certificate of AnalysisNov 04, 202411731-023, 11731023
2982260Certificate of AnalysisJul 22, 202411731-023, 11731023
2975138Certificate of AnalysisJun 17, 202411731-023, 11731023
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Safety Data Sheets

Frequently asked questions (FAQs)

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

Citations & References (1)

Citations & References
Abstract
Peroxisome Proliferator-activated Receptor gamma Agonists Inhibit HIV-1 Replication in Macrophages by Transcriptional and Post-transcriptional Effects.
Authors: Hayes Michael M; Lane Brian R; King Steven R; Markovitz David M; Coffey Michael J;
Journal:J Biol Chem
PubMed ID:11847231
Previous studies have demonstrated that cyclopentenone prostaglandins (cyPG) inhibit human immunodeficiency virus type 1 (HIV-1) replication in various cell types. We investigated the role of PG in the replication of HIV-1 in primary macrophages. The cyPG, PGA(1) and PGA(2), inhibited HIV-1 replication in acutely infected human monocyte-derived macrophages (MDM). Because ... More
1 total citations

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