Bluo-Gal

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Bluo-Gal

Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the moreRead more

Catalog Number	Quantity
15519028	1 g

Catalog number 15519028

Price (USD)

533.65

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564.00

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Quantity:

1 g

Price (USD)

533.65

Online Exclusive

564.00

Save 30.35 (5%)

Each

Add to cart

Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate. As with X-gal, Bluo-Gal produces blue (lac+) bacterial colonies and blue (lac+) viral plaques. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Bluo-Gal can also be used as a substrate with the streptavidin-β-galactosidase conjugate in both immunoblot and immunocytochemistry assays. In the presence of β-galactosidase, Bluo-Gal yields a blue precipitate, which is insoluble in alcohols and xylenes. Bluo-Gal is soluble in dimethylformamide.

Primary applications
• Blue-white screening (deeper blue as compared to X-gal)—detect functional β-galactosidase (reporter gene expression)
• Histochemical detection of β-galactosidase activity in fixed tissues
• Bluo-Gal overlay assay

Find the media format that fits your needs
Ready-to-use media formulations are available, such as LB Broth, Terrific Broth, or S.O.C recovery medium.
IPTG is available for blue-white screening.
Explore other media formats for bacterial growth.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

DescriptionBluo-Gal, Cell-Permeant

Detection MethodColorimetric

FormatBottle

MaterialHalogenated Indolyl-β-galactoside Substrate

Quantity1 g

Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice

Test TypeBlue/White Colony Screening, Immunoblotting, Immunocytochemical Assays

Unit SizeEach

Contents & Storage

• Bluo-Gal, substrate for β-galactosidase (1 g)

Store at –5°C to –30°C.

Ready-to-use Bacterial Growth Media

See how these mediums can help with critical aspects of cloning!

Gibco LB Broth ›

Invitrogen S.O.C. Medium ›

Invitrogen One Shot LB Agar* ›

*Only available in North America and selected European countries

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Frequently asked questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.