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Search Thermo Fisher Scientific
Número de catálogo | Cantidad |
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23259 | 5 Proteína |
Yes. Reducing agents interfere with this assay. Please see the list of compatibility of other commonly used reagents below:
Compatible: EDTA, Urea (<1.0M), Guanidine HCl (<1.0M), DMSO (<10%), PBS
Not Compatible: Reducing Agents, Tris, Glycerol
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Most commonly used detergents do not interfere; however, detergents with sugar derivatives, such as octyl-b-glusoside (OG) and octyl-b-thioglucopyranoside (OTG), can contribute to the signal, resulting in an erroneously high percent carbohydrate value.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Glycoprotein Carbohydrate Estimation Kit estimates the carbohydrate content of glycoproteins in solution; however, the Thermo Scientific Glycoprotein Stain directly stains oxidized glycoprotein on gels and nitrocellulose membranes and does not estimate carbohydrate content.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The kit is designed for purified proteins but can also estimate the w/w percentage of carbohydrate in total protein.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes. Free sugars form aldehydes when oxidized, which react with the Glycoprotein Detection Reagent.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
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