CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.CHAPS isRead more

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Catalog Number	Quantity
28300	5 g
28299	100 g

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Catalog number 28300

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Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

CHAPS is sulfobetaine derivative of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and is easily removed from solution by dialysis because it has a high critical micelle concentration (CMC).

Features of this buffer:

• Versatile zwitterionic detergent
• Provides mild but effective lysis of cultured mammalian cells
• Nondenaturing and generally does not inactivate protein functions
• Can be removed from solution by dialysis
• Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Applications:

• Solubilizing the opiate receptor from rat brain cell membranes, while retaining the reversible opiate binding property of the receptor ¹
• Purifying microsomal signal peptidase as a complex ²
• Solubilizing dopamine D ^2,3, muscarinic ⁴, and opiate receptors ⁵ from brain tissue

CHAPS has been successfully used to solubilize intrinsic membrane proteins and receptors and maintain the functional capability of the protein of interest. CHAPS has also been used in combination with nonionic detergents such as NP-40 for nondenaturing gel electrophoresis applications. CHAPS has largely replaced NP-40 in isoelectric focusing (IEF) applications, where it prevents streaking in certain pH ranges. The neutral charge of CHAPS and its disaggregating properties are believed to be responsible for the improvement.

Properties of CHAPS Detergent:

• Chemical Name: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
• Molecular Weight: 614.88g
• Detergent Class: Zwitterionic
• Aggregation Number: 10
• Micelle Molecular Weight: 6149g
• Critical Micelle Concentration (CMC): 8 to 10 mM (0.4920 to 0.6150%, w/v)
• Cloud Point: ≥100°C
• Dialyzable: Yes

Specifications for CHAPS:

• Visual: White free-flowing powder, free of foreign matter.
• Solubility: A 10% (aq) solution is clear, colorless.
• Identity: The I.R. scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
• Purity: (TLC) One major spot. A small, secondary spot is acceptable.

Related Products
Surfact-Amps™ Detergent Sampler

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity5 g

Reagent TypeDetergent (Pure)

FormPowder

Product TypeDetergent

Unit SizeEach

Contents & Storage

Store at Room Temperature

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Frequently asked questions (FAQs)

Are there mass spectrometry reagents for membrane proteins?

CHAPS (Cat. Nos. 28299, 28300), OTG (Cat. No. 28351) and Sodium Deoxycholate (Cat. Nos. 89904, 89905) are detergents often used to solubilize and extract membrane proteins from samples

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Are detergents denaturing or non-denaturing with respect to protein structure?

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are detergents?

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does detergent-based cell lysis work?

Detergents are amphipathic molecules, meaning they contain both a nonpolar “tail” having aliphatic or aromatic character and a polar “head”. Like the components of biological membranes, detergents have hydrophobic-associating properties as a result of their nonpolar tail groups. Nevertheless, detergents are themselves water soluble.

Consequently, detergent molecules allow the dispersion (miscibility) of water-insoluble, hydrophobic compounds into aqueous media, including the extraction and solubilization of membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What types of detergents are available for cell lysis?

Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking proteinprotein interaction. These detergents are considered harsh. Non-denaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS). These detergents do not denature proteins and do not break protein-protein interactions. These detergents are considered mild.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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