1x1 image pixel for data collection
Metal Enhanced DAB Substrate Kit
Metal Enhanced DAB Substrate Kit
Thermo Scientific™

Metal Enhanced DAB Substrate Kit

Thermo Scientific Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrateRead more
Have Questions?
Catalog NumberQuantity
34065275 mL of 1X working solution
Catalog number 34065
Price (USD)
207.65
Online Exclusive
235.00
Save 27.35 (12%)
Each
In stock
Add to cart
Quantity:
275 mL of 1X working solution
Recurring order eligible. Learn more »
Request bulk or custom format
Price (USD)
207.65
Online Exclusive
235.00
Save 27.35 (12%)
Each
Add to cart
Ask our AI about this Product
Thermo Scientific Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

Features of the Metal-Enhanced DAB Substrate Kit :

Incredible sensitivity—fifty times more sensitive than the traditional DAB method and thiry times more sensitive than other metal-intensified versions of DAB
Low background, high intensity—get a crisp dark brown-black precipitate that is more intense than the dull brown precipitate when using DAB without enhancement. And, even with the increased intensity, background is almost nonexistent
Only two components—simply mix the two liquid components and your working solution is ready to use
Six-hour stability—the innovative working solution is stable for more than six hours at room temperature, while other DAB substrates must be used immediately

To prepare the product, the 10X Metal Enhanced Substrate Solution is diluted with the Pierce Stable Peroxide Buffer prior to use, and the special formulation allows the 1X substrate to be stable for at least 6 hours at room temperature. The background color of the substrate does not increase and, unlike typical metal enhanced DAB methods, the reactivity is unchanged (1,2). The resulting precipate is a dark brown-black that is more intense than the brown color from DAB alone. The intensity not only increases, but the background is also reduced, which increases the signal-to-noise ratio and makes screening tissue or membrane antigens easier.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodChromogenic, Colorimetric
For Use With (Application)Immunodetection, IHC (Immunohistochemistry)
Product TypeDAB Substrate Kit
Quantity275 mL of 1X working solution
SubstrateDAB (Enhanced)
Substrate TypeHRP (Horseradish Peroxidase) Substrate
Product LinePierce™
Unit SizeEach
Contents & Storage
  • Metal Enhanced DAB (10X), 25 mL (store at -20°C)
  • Stable Peroxide Buffer, 250 mL (store at 4°C)
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Figures

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3213663Certificate of AnalysisJun 30, 202534065
3212003Certificate of AnalysisJun 06, 202534065
AC415163Certificate of AnalysisApr 04, 202534065
ZL401989Certificate of AnalysisFeb 12, 202534065-100UL, 34065
ZL401989ACertificate of AnalysisFeb 12, 202534065-100UL, 34065
5 results displayed, search above for a specific certificate

Safety Data Sheets

Other products to consider



Share catalog number, name or link

1x1 image pixel for data collection