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Citations & References (5)
Invitrogen™
Topoisomerase I
トポイソメラーゼI（DNA緩和酵素）は、ホスホジエステル結合の一時的な破損と再結合により、共有閉鎖性のDNAからの超らせん回転の除去を触媒します。トポイソメラーゼIはEDTAの存在下で活性化します。アプリケーション：超らせん型DNA（1）をポジティブおよびネガティブに緩和します。DNAトポイソマー詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 38042024 | 500 U |
製品番号（カタログ番号） 38042024
価格（JPY）お問い合わせください ›
111,000
Each
数量:
500 U
トポイソメラーゼI（DNA緩和酵素）は、ホスホジエステル結合の一時的な破損と再結合により、共有閉鎖性のDNAからの超らせん回転の除去を触媒します。トポイソメラーゼIはEDTAの存在下で活性化します。
アプリケーション：超らせん型DNA（1）をポジティブおよびネガティブに緩和します。DNAトポイソマー（2）の産出。
ソース：仔ウシ胸腺から浄化されます。
性能および品質検査：Endodedoxyribonuclease、3'および5'exodedoxyribonuclease、およびホスファターゼアッセイ。超らせん型DNAの緩和DNAへの変換。
ユニット定義：1つのユニットで、37℃で30分間で0.5µgの超らせん型ΦX174 RF DNAの緩和状態への変換を触媒します。
ユニット反応条件：50 mMのTris-HCl（pH 7.5）、50 mMのKCl、10 mMのMgCl2、0.1 mMのEDTA、0.5 mMのDTT、30 µg/ml/BSA、0.5 µg
ΦのX174 RF DNA、および酵素（50 µl）を37℃で30分間
アプリケーション：超らせん型DNA（1）をポジティブおよびネガティブに緩和します。DNAトポイソマー（2）の産出。
ソース：仔ウシ胸腺から浄化されます。
性能および品質検査：Endodedoxyribonuclease、3'および5'exodedoxyribonuclease、およびホスファターゼアッセイ。超らせん型DNAの緩和DNAへの変換。
ユニット定義：1つのユニットで、37℃で30分間で0.5µgの超らせん型ΦX174 RF DNAの緩和状態への変換を触媒します。
ユニット反応条件：50 mMのTris-HCl（pH 7.5）、50 mMのKCl、10 mMのMgCl2、0.1 mMのEDTA、0.5 mMのDTT、30 µg/ml/BSA、0.5 µg
ΦのX174 RF DNA、および酵素（50 µl）を37℃で30分間
研究用にのみ使用できます。診断用には使用いただけません。
仕様
製品タイプトポイソメラーゼI
数量500 U
出荷条件湿氷
酵素トポイソメラーゼ
Unit SizeEach
組成および保存条件
冷凍庫（-5～-30℃）に保存。
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証明書
Search by lot number or partial lot number
ロット番号Certificate TypeDateCatalog Number(s)
3153035Certificate of Analysis2025年5月22日38042024
3044083Certificate of Analysis2024年12月24日38042024
3037171Certificate of Analysis2024年12月09日38042024
2936440Certificate of Analysis2024年8月23日38042024
2878577Certificate of Analysis2024年4月04日38042024
5件の結果が表示されました。 上記から特定の証明書を検索します
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よくあるご質問（FAQ）
Topoisomerase I has two general applications: to relax supercoiled DNA, and to generate samples with defined superhelical density. Generating samples with defined superhelical density is described in Analytical Biochemistry, v.122, p. 253, by Singleton and Wells (1982).
For relaxing supercoiled DNA, use the following protocol:
1. Add 0.5 µg supercoiled PhiX 174 DNA, 50 mM TrisHCl (pH 7.5), 50 mM KCl, 10 mM MgCl2, 0.5 mM DTT, 0.1 mM EDTA, 30 µg/mL BSA, and 1 unit Topoisomerase I to a final volume of 50 µL.
2. Incubate 30 mins at 37 degrees C.
3. Analyze the treated samples on a 1% agarose gel that does not contain ethidium bromide. Stain with ethidium bromide after electrophoresis is complete.
No, we do not offer a pre-made buffer for use with this product.
When you edit a flask cycle, be sure to highlight a flask function in order to insert it. Similarly, when editing a cartridge cycle, highlight a cartridge function in order to insert it.
引用および参考文献 (5)
Search citations by name, author, journal title or abstract text
引用および参考文献
Abstract
Prominent mitochondrial DNA recombination intermediates in human heart muscle.
Journal:EMBO Rep
PubMed ID:11713192
'Recombination intermediates containing four-way (Holliday) junctions are generated during DNA repair and replication in many systems, including yeast mitochondrial DNA (mtDNA). In contrast, convincing evidence for recombination in mammalian mtDNA is lacking. We have used two-dimensional agarose-gel electrophoresis to analyse non-linear forms of mtDNA in human heart muscle. Replication intermediates
FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases.
Journal:Cell
PubMed ID:11832210
'FtsK acts at the bacterial division septum to couple chromosome segregation with cell division. We demonstrate that a truncated FtsK derivative, FtsK(50C), uses ATP hydrolysis to translocate along duplex DNA as a multimer in vitro, consistent with FtsK having an in vivo role in pumping DNA through the closing division
Sequence-specific trapping of topoisomerase I by DNA binding polyamide-camptothecin conjugates.
Journal:J Am Chem Soc
PubMed ID:11535069
Hairpin pyrrole-imidazole polyamides are synthetic ligands that bind in the minor groove of DNA with affinities and specificities comparable to those of DNA binding proteins. Three polyamide-camptothecin conjugates 1-3 with linkers varying in length between 7, 13, and 18 atoms were synthesized to trap the enzyme Topoisomerase I and induce
Biological characterization of MLN944: a potent DNA binding agent.
Journal:Mol Cancer Ther
PubMed ID:14749475
MLN944 (XR5944) is a novel bis-phenazine that has demonstrated exceptional efficacy against a number of murine and human tumor models. The drug was reported originally as a dual topoisomerase I/II poison, but a precise mechanism of action for this compound remains to be determined. Several lines of evidence, including the
Sticky DNA, a long GAA.GAA.TTC triplex that is formed intramolecularly, in the sequence of intron 1 of the frataxin gene.
Journal:J Biol Chem
PubMed ID:12161437
Friedreich's ataxia is caused by the massive expansion of GAA.TTC repeats in intron 1 of the frataxin (X25) gene. Our prior investigations showed that long GAA.TTC repeats formed very stable triplex structures which caused two repeat tracts to adhere to each other (sticky DNA). This process was dependent on negative
5 total citations