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Silencer™ Select Negative Control No. 1 siRNA
The Silencer™ Select Negative Control No. 1 siRNA is a non-targeting negative control siRNA with the same chemical modifications forRead more
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| Catalog Number | Quantity |
|---|---|
| 4390843 | 5 nmol |
| 4390844 | 40 nmol |
Catalog number 4390843
Price (USD)
253.80
Special offer
Online exclusive
282.00Save 28.20 (10%)
Each
In stock
Quantity:
5 nmol
Price (USD)
253.80
Special offer
Online exclusive
282.00Save 28.20 (10%)
Each
The Silencer™ Select Negative Control No. 1 siRNA is a non-targeting negative control siRNA with the same chemical modifications for enhanced efficacy as in other Silencer™ Select siRNAs. Properties of Silencer™ Select control siRNAs:
• Validated siRNA controls for optimizing siRNA experiments
• GAPDH Positive Control functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer™ Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer™ Select siRNAs?
Silencer™Select siRNAs are our next-generation siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Silencer™ Select Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. We have designed and extensively tested two Silencer™ Select Negative Control siRNAs. These siRNAs include the same modifications for reducing off-target effects as found in other Silencer™ Select siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these two controls have been tested in multi-parametric, cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.
Quality control
We synthesize and purify each Silencer™ Select siRNA in state-of-the-art facilities to meet the highest-quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
• Validated siRNA controls for optimizing siRNA experiments
• GAPDH Positive Control functionally tested in several common cell lines
• Negative Controls functionally proven to have minimal effects on cell proliferation and viability
• Include Silencer™ Select modifications for enhanced specificity
• For use in human, mouse, and rat cells
What are Silencer™ Select siRNAs?
Silencer™Select siRNAs are our next-generation siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
Silencer™ Select Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. We have designed and extensively tested two Silencer™ Select Negative Control siRNAs. These siRNAs include the same modifications for reducing off-target effects as found in other Silencer™ Select siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these two controls have been tested in multi-parametric, cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.
Quality control
We synthesize and purify each Silencer™ Select siRNA in state-of-the-art facilities to meet the highest-quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)RNAi, Transfection
Label or DyeUnconjugated
Product LineSilencer™, Silencer™ Select, Ambion™
Product TypesiRNA
PurityHPLC
Quantity5 nmol
Shipping ConditionRoom Temperature
Control TypeNegative Control
RNAi TypesiRNA
Unit SizeEach
Contents & Storage
Contains dried siRNA at (5 nmol) and Nuclease-free Water (1.75 mL), shipped at ambient temperature. Upon receipt, store siRNAs in a non-frost-free freezer at or below –20 °.
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'
Notice to Purchaser: This product is licensed from Alnylam Pharmaceuticals, Inc., Cambridge, USA and is provided only for use in academic and commercial research, including research to validate potential gene products and pathways for drug discovery and development and to screen non-siRNA based compounds and not for any other commercial purposes. Information about licenses for commercial use (including development of siRNA-based drugs) is available from Alnylam Pharmaceuticals, Inc., 300 Third Street, Cambridge, MA 02142, USA.
'Frequently asked questions (FAQs)
Vector technologies allow you to:
Achieve transient or stable target knockdown
Perform RNAi in any cell type, even hard-to-transfect, primary, and non-dividing cells
Regulate gene inhibition with inducible siRNA expression
Select for a pure population of cells stably expressing an siRNA sequence
Control gene expression in vivo with tissue-specific promoters
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
We would suggest running a transfection reagent control only to determine if your cells are sensitive to the transfection reagent. Additionally, you can try using different cell densities and siRNA concentrations to diminish any toxic effects from the transfection itself.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
In some cases, knockdown of a protein can be affected by other variables such as protein turnover rate, even though the RNA is knocked down. Additionally, a longer time course may be needed to see an effect on protein compared to mRNA.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Please see the following possibilities and suggestions:
- How many siRNA did you test? Is there any knockdown? If there is no knockdown (<10%) in any of the siRNA, then the assay is likely the problem. Try using a different qRT-PCR assay to assess knockdown.
- What was the positioning of the qRT-PCR assay target site relative to the cut site for the siRNA? If greater than 3,000 bases away, the problem could be alternative splice transcripts.
- What are the Cts for the experiment? They should be below 35 in a 40-cycle qRT-PCR experiment.
- Did you confirm the siRNA got into the cell? We recommend using a validated positive control siRNA to check the transfection efficiency.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
Please see the following possibilities and suggestions:
- Were the mRNA levels checked? The most reliable method is real-time PCR. In some cases, knockdown of a protein can be affected by other variables, such as protein turnover rate, even though the RNA is knocked down.
- How is the RNA being isolated? Has the quality of the isolated RNA been checked? Ensure that the RNA has not been degraded.
- Was a positive control used? This can help to determine whether the reagents are working and whether the siRNA was delivered correctly to the cell. Run your experiment in parallel with the positive control siRNA.
- Was a transfection control used? What is the percentage of transfected cells?
- Was a time course used? Generally, gene silencing can be assessed as early as 24 hours posttransfection. However, the duration and level of knockdown are dependent on cell type and concentration of siRNA.
- Was optimization of transfection conditions performed? You can try using different cell densities and siRNA concentrations.
- Which concentration of siRNA did you use? We recommend testing multiple concentrations between 5 nM and 100 nM.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.