Pierce™ Protein A Magnetic Beads
Pierce™ Protein A Magnetic Beads
Thermo Scientific™

Pierce™ Protein A Magnetic Beads

これらの高容量かつハイスループットのアフィニティービーズを使用して、手動またはロボットの磁気分離器による抗体精製および免疫沈降法を行えます。
製品番号(カタログ番号)数量
888451 mL
888465 mL
製品番号(カタログ番号) 88845
価格(JPY)
35,200
Each
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数量:
1 mL
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Thermo Scientific Pierce Protein A Magnetic Beads are high-capacity and high-throughput affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A Magnetic Beads:

  • High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers
  • Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)
  • Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation
  • Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts. Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments. The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific KingFisher Instruments, for high-throughput workflows.

Applications:

  • IP and Co-IP experiments
  • Antibody purification

Thermo Scientific Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

研究用途にのみご使用ください。診断目的には使用できません。
Thermo Scientific™ Pierce Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co–IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Pierce Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.
仕様
濃度Slurry: 10mg/mL, 1% solids
リガンドタイプProtein A
タンパク質形態Recombinant
数量1 mL
ターゲットAntibody
容量(メートル法)1 mL
形状Liquid
粒子径1 μm
製品ラインPierce™
タイプMagnetic Affinity Bead
Unit SizeEach
組成および保存条件
受け取り後4°Cで保存。製品はアイスパック入りで出荷されます。
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