GeneChip™ Human Genome U133 Plus 2.0 Array
GeneChip™ Human Genome U133 Plus 2.0 Array
Applied Biosystems™

GeneChip™ Human Genome U133 Plus 2.0 Array

GeneChip™ Human Genome U133 Plus 2.0 ArrayBenefits of the first and most comprehensive whole human genome expression array• Convenient one-arrayRead more
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Catalog NumberNumber of Arrays
90046730 arrays
9004666 arrays
Catalog number 900467
Price (USD)
-
Number of Arrays:
30 arrays
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GeneChip™ Human Genome U133 Plus 2.0 Array

Benefits of the first and most comprehensive whole human genome expression array
• Convenient one-array view.
• Complete coverage of the Human Genome U133 Set plus 6,500 additional genes for analysis of over 47,000 transcripts.
• Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform.
• GeneChip System: Richer data, sharper insights, better decisions

Array Profile
All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. The sequences from which these probe sets were derived were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

In addition, there are 9,921 probe sets representing approximately 6,500 genes. These gene sequences were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from the UniGene database (Build 159, January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
TypeHuman Genome U133 Plus 2.0 Array
ArrayTranscriptome Profiling
Number of Arrays30 arrays
FormatArray Cartridge
SpeciesHuman
Product LineGeneChip™
Quantity30 arrays
Unit SizeEach
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Frequently asked questions (FAQs)

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

The suffixes, at the end of each probeset in GeneChip 3' IVT arrays, indicate what is known about the sequence at the time of design:
_at = all the probes hit one known transcript
_a = all probes in the set hit alternate transcripts from the same gene
_s = all probes in the set hit transcripts from the different genes
_x = some probes hit transcripts from different genes

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Yes, but we recommend storing your GeneChip 3' IVT Array after hybridization and prior to the fluidics step, for no longer than 3 hours at 4 degrees C. Please remove the hybridization cocktail and fill with wash A before storing.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

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