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CD OptiCHO™ AGT™ Medium
CD OptiCHO™ AGT™ Medium
Gibco™

CD OptiCHO™ AGT™ Medium

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically definedRead more
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Catalog NumberQuantity
A11222041 L
A112220510 L
A1122201100 L
Catalog number A1122204
Price (USD)
131.00
Each
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1 L
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Price (USD)
131.00
Each
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CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco™ CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.

When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky,” i.e. hard to grow or adapt

When to Consider Another Gibco™ Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco™ AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco™ liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineCHO
Culture EnvironmentCO2
Endotoxin LevelLow
FormatSecuritainer Capped Vial
Included AntibioticsNo antibiotics
Inorganic SaltsMagnesium, Sodium Phosphate
Product LineAGT™, Gibco™
Product TypeCD OptiCHO AGT Medium
Purity or Quality GradeCertified
Quantity1 L
Reconstituted Volume1 L
RemarksMedium does not support wild type DG44 cells
Shipping ConditionWet Ice
ClassificationAnimal Origin-free, Chemically-defined, Protein-free, Serum-free
Culture TypeSuspension Cell Culture
FormPowder (AGT)
Serum LevelSerum-free
SterilityNon-sterile
With AdditivesHigh Glucose, Sodium Pyruvate, Sodium Bicarbonate
Without AdditivesNo Glutamine, No Phenol Red
Unit SizeEach
Contents & Storage
1 x 1 L, Storage 2-8°C Dark and Dry
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Frequently asked questions (FAQs)

What are the main benefits of using AGT?

Complete formulations
- Homogeneous distribution of minute components
- Complete complex formulations in a dry format, including serum-free, protein-free, and chemically defined nutrient media
- Pre-adjusted to the appropriate pH and osmolality
- Require only standard supplementation with L-glutamine for L-glutamine dependent systems
- Can be customized for modified catalog and customers' proprietary formulations

Granular format
- Dissolves instantly for faster media preparation times
- Dust-free granules reduce mess and environmental contamination
- Performance equivalent to liquid media
- Workflow improvement
- Helps reduce total cycle costs due to lower number of components, decreasing time involved in raw material planning, procurement, and testing
- Faster and easier media preparation, with less potential for error
- Easy to scale up from research through production, in batch sizes from 2 kg to 6,000 kg
- Available in various packaging options

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is AGT?

Granulated cell culture media is an easy-to-use, granular form of dry media that is produced by applying fluid bed granulation technology to cell culture nutrient media manufacturing. The patented process allows production of complete formulations of a variety of serum-free, protein-free, and chemically defined media in a dry format.

AGT offers the benefits of liquid media without the cost, storage, and transportation issues. It is a complete medium that is pH and osmolality pre-adjusted. The granules dissolve instantly for faster media preparation time than conventional dry powder media. AGT media can therefore help reduce total cycle costs, decreasing time involved in raw material planning, procurement, and testing as well as media preparation. These benefits are all realized while maintaining comparable cell growth to liquid media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3232040Certificate of AnalysisJun 22, 2025A1122201, A1122205, A1122204, A1122203
3232019Certificate of AnalysisJun 22, 2025A1122201, A1122205, A1122204, A1122203
3232757Certificate of AnalysisJun 01, 2025A1122201, A1122205, A1122204, A1122203
3232743Certificate of AnalysisJun 01, 2025A1122201, A1122205, A1122204, A1122203
3187550Certificate of AnalysisMay 22, 2025A1122201, A1122205, A1122204, A1122203
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Safety Data Sheets

Limited Use Label Licenses (LULL)

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Notice to Purchaser: This product is covered by US and ex-US patents owned by Life Technologies, corresponding to chemically defined cell culture media and uses thereof. The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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