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Viral Production Cells 2.0
Viral Production Cells 2.0
Gibco™

Viral Production Cells 2.0

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a coreRead more
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Catalog NumberFormat
A497841 vial
A512186 vials
Catalog number A49784
Price (USD)
1,516.00
Each
In stock
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Format:
1 vial
Recurring order eligible. Learn more »
Price (USD)
1,516.00
Each
Add to cart
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Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System. The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium. Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production.

Features of the Viral Production Cells 2.0 include:
• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
• Robust scalability and passage stability

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO. Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell StateFrozen
Concentration1 x 107 cells/mL
For Use With (Application)AAV Production
Format1 vial
Media RecommendationGibco Viral Production Medium
No. of Cells1 x 107 cells
Product LineAAV-MAX
Product TypeViral Production Cells
Quantity1 vial
Shipping ConditionDry ice for domestic shipment; liquid nitrogen for international shipment
SourceHuman
Cell LineViral Production Cells 2.0
Cell TypeKidney (Embryonic)
Culture TypeSuspension Cell Culture
FormFrozen
SpeciesHuman
Unit SizeEach
Contents & Storage
1 cryovial, store in vapor phase liquid nitrogen
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Frequently asked questions (FAQs)

Can Viral Production Cell 2.0 (Cat. No. A49784, A51218) only grow in suspension, or can they be adherent using specific medium conditions?

Viral Production Cells 2.0 were designed specifically for suspension cultures. We did not validate their usage with adherent culture and therefore do not recommend doing so.

Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?

The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.

What is the highest density that Viral Production Cells 2.0 can be cultured at?

The Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.

What is the passage stability of the Viral Production Cells 2.0?

We recommend using the Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.

How long do the Viral Production Cells 2.0 require to recover post-thaw before transfection?

We recommend allowing freshly thawed Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3043918Certificate of AnalysisFeb 03, 2025A49784
2877843Certificate of AnalysisJul 11, 2024A49784
2661435Certificate of AnalysisAug 15, 2023A49784
2561979Certificate of AnalysisJun 05, 2023A49784
2499725Certificate of AnalysisSep 07, 2022A49784
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Safety Data Sheets

Limited Use Label Licenses (LULL)

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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser's activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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