Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System. The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium. Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production.

Features of the Viral Production Cells 2.0 include:
• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
• Robust scalability and passage stability

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Viral Production Medium and 10% DMSO. Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance.