Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

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Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

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Catalog Number	Quantity	Product Type
A72127	100 Reactions	Lyo-ready RPA Kit
A72123	1600 μg	Lyo-ready T4 Gene 32 Protein
A72124	120 μg	Lyo-ready T4 UvsX Protein
A72125	120 μg	Lyo-ready T4 UvsY Protein
A72126	2000 μL	Reaction Buffer
A72128	500 Reactions	Lyo-ready RPA Kit

6 Options

Catalog number A72127

Price (USD)

282.00

Each

In stock

Add to cart

Quantity:

100 Reactions

Product Type:

Lyo-ready RPA Kit

Price (USD)

282.00

Each

Add to cart

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
Robust and specific RNA/DNA amplification
Amplification from inhibitor-containing samples
Fast target amplification in as little as 20 minutes
Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
Optimized reaction buffer, magnesium chloride, and dNTPs

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodEnd point, real-time, lateral flow

Optimal Reaction Temperature42°C

Reaction TimeUp to 20 min.

Reverse TranscriptaseSuperScript IV Reverse Transcriptase

Shipping ConditionDry Ice

For Use With (Application)RPA, RT-RPA

FormLiquid

PolymeraseLyo-ready Bst DNA Polymerase

Product TypeLyo-ready RPA Kit

Quantity100 Reactions

Research CategoryNucleic Acid Amplification

Unit SizeEach

Contents & Storage

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl₂, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.

Enhance RPA Results with Lyo-ready Bst DNA Polymerase

Try Lyo-Ready Bst DNA Polymerase for rapid and sensitive detection of various pathogens.

Discover Recombinase Polymerase Amplification (RPA)

Simple, sensitive, and specific DNA amplification using RPA technology.

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Frequently asked questions (FAQs)

Can I use PCR primers for target amplification by the Lyo-ready RPA Kit (Cat. No. A72127, A72128)?

PCR primers can be used with the Lyo-ready RPA Kit if they are at least 30 nt long. RPA primers can be designed using Primer3.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I lyophilize reagents from the Lyo-ready RPA Kit?

All RPA reagents (including Lyo-ready T4 UvsX Protein (Cat. No. A72124), Lyo-ready T4 UvsY Protein (Cat. No. A72125), Lyo-ready T4 Gene 32 Protein (Cat. No. A72123), Lyo-ready Bst DNA Polymerase (Cat. No. A56655), and RPA Reaction Buffer (2X) (Cat. No. A72126)) are lyo-ready, ensuring that enzymes and buffer formulations are glycerol-free. However, the formulations do not contain excipients needed for the lyophilization process.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

I noticed that my Lyo-ready RPA Kit is frozen after storage at -20 degrees C. Is it still good to use?

Lyo-ready RPA Kit and stand-alone reagents come in a storage buffer that does not contain glycerol. Product freezing doesn't affect its performance or stability. We do not recommend freeze-thawing Lyo-ready RPA Kit and stand-alone reagents for more than 20 cycles.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What is the temperature limit to perform an RPA reaction using a Lyo-ready RPA Kit (Cat. No. A72127, A72128)?

Lyo-ready RPA Kit can amplify DNA in temperatures ranging from 37 to 45 degrees C. To ensure the best performance, we recommend using the optimal reaction temperature of 42 degrees C.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I use my reverse transcriptase with a Lyo-ready RPA Kit (Cat. No. A72127, A72128) for RT-RPA?

We recommend using SuperScript IV Reverse Transcriptase (Cat. No. 18090010, 18090050, 18090200) for the best results in RT-RPA. Guidelines for use of SuperScript IV Reverse Transcriptase in RT-RPA application can be found in the Lyo-ready RPA Kit user guide. Other reverse transcriptases such as Maxima H Minus Reverse Transcriptase (Cat. No. EP0751) have also been tested and can be used in RT-RPA application. The list of Lyo-ready reverse transcriptases can be found here: thermofisher.com/lyo-ready.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA) FAQs