Search Thermo Fisher Scientific
Certificates
SDS
Thermo Scientific™
phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficientRead more
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| EP0091 | 250 units |
| EP0092 | 1,000 units |
| EP0094 | 5,000 units |
Catalog number EP0091
Price (EUR)
103,00
Each
-
Quantity:
250 units
Price (EUR)
103,00
Each
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. Produced from E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.
Features of phi29 DNA Polymerase
• Excellent processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Highly accurate DNA synthesis
• High yields of amplified DNA even from small amounts of template
Applications
Phi29 DNA Polymerase may be used in a wide variety of applications including:
• Rolling circle amplification (RCA)—generation of periodic DNA nanotemplates
• Multiple displacement amplification (MDA)
• Unbiased amplification of whole genome (WGA) (see figure below)
• Amplification of DNA for SNP and STR detection
• Cell-free amplification of DNA from single cells, pathogenic organisms, or metagenomes
• DNA template preparation for sequencing
• Protein-primed DNA amplification
• Recombination based-cloning
• Cell-free cloning of lethal DNA
• RNA-primed DNA amplification
Notes
1. The addition of pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.
2. Because of the enzymes’s 3’→5’ proofreading exonuclease, 3'-modified primers are highly recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 U/μL
Optimal Reaction Temperature30°C
Reaction Time4 hours
Shipping ConditionDry Ice
For Use With (Application)MDA-WGA, RCA
Polymerasephi29 DNA Polymerase
Product TypeStand-alone enzyme
Quantity250 units
Unit SizeEach
Contents & Storage
• phi29 DNA Polymerase (250 units at 10 U/μL)
• 10X Reaction Buffer for phi29 DNA Polymerase
• Store at –15 to –25°C.
• 10X Reaction Buffer for phi29 DNA Polymerase
• Store at –15 to –25°C.
Discover Rolling Circle Amplification (RCA) and Whole Genome Amplification (WGA)
Fast, sensitive, and effective nucleic acid amplification. Find out more about RCA and WGA technology ›
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Figures
Unbiased amplification of human genomic DNA with phi29 DNA Polymerase
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3247225Certificate of AnalysisMay 26, 2025EP0094
3245947Certificate of AnalysisMay 26, 2025EP0092
3240258Certificate of AnalysisMay 15, 2025EP0091
3203609Certificate of AnalysisApr 04, 2025EP0094
3198809Certificate of AnalysisMar 27, 2025EP0091
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
The error rate of phi29 DNA Polymerase is 1 x 10-5.
The error rate of phi29 DNA Polymerase was measured according to the method described in literature:
Mielinis, P., Sukackaitė, R., Serapinaitė, A., Samoilovas, F., Alzbutas, G., Matjošaitis, K., & Lubys, A. (2021). MUA-based molecular indexing for rare mutation detection by Next-Generation sequencing. Journal of Molecular Biology, 433(19), 167209. https://doi.org/10.1016/j.jmb.2021.167209
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
phi29 DNA Polymerase should be able to incorporate 5-methyl-dCTP nucleotides and other modified nucleotides.
The incorporation of modified nucleotides by phi29 DNA Polymerase can be reviewed in the literature:
Jakubovska, J., Tauraitė, D., Birštonas, L., & Meškys, R. (2018). N 4-acyl-2′-deoxycytidine-5′-triphosphates for the enzymatic synthesis of modified DNA. Nucleic Acids Research, 46(12), 5911–5923. https://doi.org/10.1093/nar/gky435
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
The optimal reaction time for DNA amplification with phi29 DNA Polymerase is 4 hours. For samples with ≥1 pg of DNA input, DNA amplification time can be shortened to 2 hours if maximizing product yield is not essential.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Yes. phi29 DNA Polymerase can work with different types of sample input such as purified DNA, liquid media culture, agar plate colonies, etc.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Yes, we recommend heat-inactivating phi29 DNA Polymerase at 65 degrees C for 10 min.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.