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Invitrogen™
pYES2.1 TOPO™ TA Yeast Expression Kit
The pYES2.1 TOPO TA Expression Kit offers direct cloning of a Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector.Read more
| Catalog Number | Quantity |
|---|---|
| K415001 | 20 reactions |
Catalog number K415001
Price (USD)
1,330.00
Each
In stock
Quantity:
20 reactions
Price (USD)
1,330.00
Each
The pYES2.1 TOPO TA Expression Kit offers direct cloning of a Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector. The kit uses the linearized, topoisomerase I-activated pYES2.1/V5-His-TOPO ¤ vector for 5-minute cloning on your bench top and produces >85% recombinants. The pYES2.1/V5- His-TOPO™ vector features:
• 2μ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal media
• C-terminal V5 epitope and polyhistidine (6xHis) tags for efficient detection and purification
• 2μ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal media
• C-terminal V5 epitope and polyhistidine (6xHis) tags for efficient detection and purification
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialAmpicillin (AmpR)
Product TypeTA Yeast Expression Kit
Quantity20 reactions
VectorpYES, TOPO-TA Cloning Vectors
Cloning MethodTOPO-TA
Product LineTOPO™, YES™
PromoterGAL1
Protein TagHis Tag (6x), V5 Epitope Tag
Unit SizeEach
Contents & Storage
Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 μl aliquots of chemically competent TOP10F´ E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored.
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Citations & References (7)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Identification of a family of animal sphingomyelin synthases.
Journal:EMBO J
PubMed ID:14685263
'Sphingomyelin (SM) is a major component of animal plasma membranes. Its production involves the transfer of phosphocholine from phosphatidylcholine onto ceramide, yielding diacylglycerol as a side product. This reaction is catalysed by SM synthase, an enzyme whose biological potential can be judged from the roles of diacylglycerol and ceramide as
Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability.
Journal:Mol Cell Biol
PubMed ID:10373538
'In Saccharomyces cerevisiae, the heteromeric kinase complex Cdc7p-Dbf4p plays a pivotal role at replication origins in triggering the initiation of DNA replication during the S phase. We have assayed the kinase activity of endogenous levels of Cdc7p kinase by using a likely physiological target, Mcm2p, as a substrate. Using this
Regulation of stress response signaling by the N-terminal dishevelled/EGL-10/pleckstrin domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae.
Journal:J Biol Chem
PubMed ID:11940600
'All members of the regulator of G protein signaling (RGS) family contain a conserved core domain that can accelerate G protein GTPase activity. The RGS in yeast, Sst2, can inhibit a G protein signal leading to mating. In addition, some RGS proteins contain an N-terminal domain of unknown function. Here
Genetic screens in yeast to identify mammalian nonreceptor modulators of G-protein signaling.
Journal:Nature Biotechnology
PubMed ID:10471929
We describe genetic screens in Saccharomyces cerevisiae designed to identify mammalian nonreceptor modulators of G-protein signaling pathways. Strains lacking a pheromone-responsive G-protein coupled receptor and expressing a mammalian-yeast Galpha hybrid protein were made conditional for growth upon either pheromone pathway activation (activator screen) or pheromone pathway inactivation (inhibitor screen). Mammalian
Pheromone-dependent Ubiquitination of the Mitogen-activated Protein Kinase Kinase Ste7.
Journal:J Biol Chem
PubMed ID:11864977
Many cell signaling pathways are regulated by phosphorylation, ubiquitination, and degradation of constituent proteins. As with phosphorylation, protein ubiquitination can be reversed, through the action of ubiquitin-specific processing proteases (UBPs). Here we have analyzed 15 UBP disruption mutants in the yeast Saccharomyces cerevisiae and identified one (ubp3Delta) that acts specifically
7 total citations