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Citations & References (62)
Invitrogen™
XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)
XTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble XTTRead more
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| Catalog Number | Quantity |
|---|---|
| X6493 | 100 mg |
Catalog number X6493
Price (USD)
254.00
100 mg
In stock
Quantity:
100 mg
Price (USD)
254.00
100 mg
XTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble XTT to a water-soluble, orange colored formazan product. Unlike MTT, XTT does not require solubilization prior to quantitation, thereby reducing the assay time in many viability assay protocols. Moreover, the sensitivity of the XTT reduction assay is reported to be similar to or better than that of the MTT reduction assay.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEukaryotic Cells
Detection MethodColorimetric
Dye TypeOther Label(s) or Dye(s)
Format96-well plate
Quantity100 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Microplate Reader
Product TypeXTT
Unit Size100 mg
Contents & Storage
Store in freezer -5°C to -30°C.
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Lot #Certificate TypeDateCatalog Number(s)
3023515Certificate of AnalysisOct 25, 2024X6493
2861863Certificate of AnalysisFeb 07, 2024X6493
2761137Certificate of AnalysisAug 18, 2023X6493
2720257Certificate of AnalysisJul 14, 2023X6493
2570541Certificate of AnalysisApr 12, 2023X6493
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Both products use the same reagent, XTT. In theory, the results of any optimized assay using XTT (Cat. No. X6493) may be comparable to using the CyQUANT XTT Cell Viability Assay kit (Cat. No. X12223). Variations may arise due to the type of electron coupling reagent used, the final concentration of XTT, and any assay-specific requirements of the solutions used in resolubilizing the reagents.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
For the CyQUANT XTT Cell Viability Assay kit (Cat No. X12223), the amount of reagents, the formulation of the solutions, and the identity of the Electron Coupling Reagent are proprietary information. On the other hand, XTT (Cat. No. X6493) may be resolubilized in either water, buffer, media, or DMSO to a desired stock concentration. We would recommend using XTT, Cat No. X6493 if you need to optimize the final working concentration, if the type of buffer/solvent used to resolubilize the reagent is critical to the study, if you elect not to use an electron coupling reagent, or if you'd like to use a known electron coupling reagent of a defined concentration.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
XTT (Cat No. X6493) is 100 mg of XTT in solid form; you must resolubilize this into an aqueous solution or DMSO prior to use.
CyQUANT XTT Cell Viability Assay (Cat No. X12223) is a kit that provides two components: 10 vials each of the XTT Reagent and Electron Coupling Reagent. Both kit components are in solution in a ready-to-use format; mix 1 vial of the XTT Reagent and 1 vial of the Electron Coupling Reagent immediately before use.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (62)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Endoglin is a component of the transforming growth factor (TGF)-beta receptor complex of human pre-B leukemic cells.
Journal:J Immunol
PubMed ID:8543807
'Endoglin was first identified on a cell line derived from pre-B acute lymphoblastic leukemia. This 180-kDa homodimeric glycoprotein was then shown to be primarily expressed on endothelial cells and to bind the beta 1 and beta 3 isoforms of TGF-beta with high affinity. We now demonstrate that pre-B leukemic cells
9-Nitrocamptothecin inhibits HIV-1 replication in human peripheral blood lymphocytes: a potential alternative for HIV-infection/AIDS therapy.
Journal:J Med Virol
PubMed ID:11424110
'The ability of the anti-cancer drug, 9-Nitrocamptothecin (9NC), to inhibit replication of HIV-1 in clinically relevant primary lymphocytic cells was studied. Primary peripheral blood lymphocytes (PBLs) from a non-infected donor were freshly infected with HIV-1 and treated with 9NC by using three different treatment schedules. Cells were monitored for cytotoxicity
In vitro effects of antiviral agents on human keratocytes.
Journal:Cornea
PubMed ID:11189008
'PURPOSE: To study the effects of antiviral agents on human keratocytes in vitro. METHODS: Cultured human keratocytes were incubated with either ganciclovir, idoxuridine, trifluridine, or cidofovir at concentrations from 0.0001 to 10 mg/mL. Phase-contrast microscopy and XTT (sodium [2,3-bis [2-methoxy-4-nitro-5-sulphophenyl]-2h-tetrazolium-5-carboxanilide, inner salt) colorimetric assay were performed after 24, 48, and
The interaction of human monocytes, monocyte-derived macrophages, and polymorphonuclear neutrophils with caspofungin (MK-0991), an echinocandin, for antifungal activity against Aspergillus fumigatus.
Journal:Diagn Microbiol Infect Dis
PubMed ID:11248522
'The collaboration between human effector cells and caspofungin (MK-0991), a 1,3-beta-D glucan synthase inhibitor, was studied for antifungal activity against Aspergillus fumigatus. Caspofungin was co-cultured for 24h with either human monocytes (Monos), monocyte-derived macrophages (MDM), or polymorphonuclear neutrophils (PMN) against germlings of A. fumigatus and antifungal activity assessed using the
In vitro growth and analysis of Candida biofilms.
Journal:Nat Protoc
PubMed ID:19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion
62 total citations