Zenon™ Mouse IgG1 Labeling Kits
New Zenon Alexa Fluor Plus Labeling Reagents are superior in three ways: improved bioconjugation for brighter signal, redesigned protein for robust screening regardless of IgG isotype, and new, more sensitive fluorophores.
Zenon&trade; Mouse IgG<sub>1</sub> Labeling Kits
Invitrogen™

Zenon™ Mouse IgG1 Labeling Kits

Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits.
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Catalog NumberQuantityExcitation/EmissionLabel or Dye
Z25055
also known as Z-25055
25 Reactions kit496, 546, 565/578 nmR-PE (R-Phycoerythrin)
Z2500250 Reactions kit496/519 nmAlexa Fluor 488
Z2500550 Reactions kit555/565 nmAlexa Fluor 555
Z25006
also known as Z-25006
50 Reactions kit578/603 nmAlexa Fluor 568
Z2500750 Reactions kit590/617 nmAlexa Fluor 594
Z2500850 Reactions kit650/668 nmAlexa Fluor 647
Z2501150 Reactions kit696/719 nmAlexa Fluor 700
Z2501350 Reactions kit402/421 nmAlexa Fluor 405
Z25051
also known as Z-25051
25 Reactions kit650/660 nmAPC (Allophycocyanin)
Z25052
also known as Z-25052
50 Reactions kitBiotin
Catalog number Z25055
also known as Z-25055
Price (USD)
530.00
1 kit
-
Add to cart
Quantity:
25 Reactions kit
Excitation/Emission:
496, 546, 565/578 nm
Label or Dye:
R-PE (R-Phycoerythrin)
Recurring order eligible. Learn more »
Price (USD)
530.00
1 kit
Add to cart
Ask our AI about this Product
Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG1 labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG1 primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.

Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:
• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 μg primary antibody
• Simple—no purification required
• Flexible—choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 μg in 2 μL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.

Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.

Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.

Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.

Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.

Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed-Orange
Excitation/Emission496, 546, 565/578 nm
Label TypePE & APC
Labeling Scale< 1–20 μg
Product LineZenon™
Quantity25 Reactions kit
SpeciesMouse
Labeling TargetIgG1
Label or DyeR-PE (R-Phycoerythrin)
Unit Size1 kit
Contents & Storage
Contains 1 vial of Zenon R-PE mouse IgG1 labeling reagent (125 μL), and 1 vial of Zenon blocking reagent (mouse IgG, 125 μL).

Store in refrigerator (2–8°C) and protect from light.
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Lot #Certificate TypeDateCatalog Number(s)
3154558Certificate of AnalysisMar 31, 2025Z25013
3109187Certificate of AnalysisNov 25, 2024Z25002
2956390Certificate of AnalysisOct 09, 2024Z25052
2936208Certificate of AnalysisJul 15, 2024Z25055
2896459Certificate of AnalysisJun 24, 2024Z25008
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Citations & References (67)

Citations & References
Abstract
CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
Authors:Kawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, Kawana Y, Yang H, Marrero L, Yavagal S, Greene SJ, Zhang YX, Pyles RB, Blumberg RS, Schust DJ
Journal:J Biol Chem
PubMed ID:17215251
'Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we ... More
Rapid visualization of microtubules in blood cells and other cell types in marine model organisms.
Authors:Lee KG, Braun A, Chaikhoutdinov I, DeNobile J, Conrad M, Cohen W
Journal:Biol Bull
PubMed ID:12414579
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
Authors:Duret C, Gerbal-Chaloin S, Ramos J, Fabre JM, Jacquet E, Navarro F, Blanc P, Sa-Cunha A, Maurel P, Daujat-Chavanieu M
Journal:Stem Cells
PubMed ID:17412893
'Activation and proliferation of human liver progenitor cells has been observed during acute and chronic liver diseases. Our goal was to investigate the presence of these putative progenitors in the liver of patients who underwent lobectomy for various reasons but did not show any hepatic insufficiency. Hepatic lesions were evaluated ... More
JNK phosphorylates synaptotagmin-4 and enhances Ca2+-evoked release.
Authors:Mori Y, Higuchi M, Hirabayashi Y, Fukuda M, Gotoh Y,
Journal:EMBO J
PubMed ID:18046461
'Ca2+ influx induced by membrane depolarization triggers the exocytosis of secretory vesicles in various cell types such as endocrine cells and neurons. Peptidyl growth factors enhance Ca2+-evoked release, an effect that may underlie important adaptive responses such as the long-term potentiation of synaptic transmission induced by growth factors. Here, we ... More
Cocaine-induced dendritic spine formation in D1 and D2 dopamine receptor-containing medium spiny neurons in nucleus accumbens.
Authors:Lee KW, Kim Y, Kim AM, Helmin K, Nairn AC, Greengard P
Journal:Proc Natl Acad Sci U S A
PubMed ID:16492766
'Psychostimulant-induced alteration of dendritic spines on dopaminoceptive neurons in nucleus accumbens (NAcc) has been hypothesized as an adaptive neuronal response that is linked to long-lasting addictive behaviors. NAcc is largely composed of two distinct subpopulations of medium-sized spiny neurons expressing high levels of either dopamine D1 or D2 receptors. In ... More
67 total citations

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