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Applied Biosystems™
BigDye™ Terminator v3.1 Cycle Sequencing Kit
Catalog number: 4337454
Have Questions? 
Applied Biosystems™
BigDye™ Terminator v3.1 Cycle Sequencing Kit
Catalog number: 4337454
Have Questions? | Catalog Number | Unit Size | Price (USD) | |
|---|---|---|---|
| 4337454 | 24 reactions | Request A Quote | |
| 4337455 | 100 reactions | Request A Quote | |
| 4337456 | 1,000 reactions | Request A Quote | |
| 4337457 | 5,000 reactions | Request A Quote | |
| 4337458 | 25,000 reactions | Request A Quote |
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The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
- Improve the quality of your results for a wide range of sequencing applications
- Optimized for long read lengths
- Better dye mobility characteristics
- Improved performance reading through GT-rich regions
- Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
- Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format
12-well Strips, 8-well Strip, 96- or 384-well Plate
For Use With (Equipment)
SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
For Use With (Application)
AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
Product Line
BigDye™ Terminator
Product Type
Sequencing Buffer
Quantity
24 Reactions
Technique
Fluorescent Dye Terminator Sequencing
Template Compatibility
BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons
Contents & Storage
• 200 μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix
• 1 tube M13 (-21) Primer
• 1 tube pREF-BDT™ Control DNA
• 1 mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
• 1 tube M13 (-21) Primer
• 1 tube pREF-BDT™ Control DNA
• 1 mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
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