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Invitrogen™
ChargeSwitch™ PCR Clean-Up Kit
Catalog number: CS12000
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ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.
• Sample size: 25–50 μL
• High recovery of DNA fragments
• Rapid, one tube, 5 minute protocol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format
Kit
High-throughput Compatibility
High-throughput Compatible
Product Line
ChargeSwitch™
Product Type
PCR Clean-Up Kit
Quantity
100 Preps
Run Time
5 min
Shipping Condition
Room Temperature
Target
DNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)
Next-Generation Sequencing (NGS), Cloning, Standard PCR
Isolation Technology
ChargeSwitch™ Chemistry
No. of Reactions
100 Preps
Sample Type
PCR Products
Contents & Storage
The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.
Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
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