Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits
Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits
Invitrogen™

Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits

Antibody Labeling Kits specifically bind small amounts of antibody with violet light-excitable Pacific Orange or Pacific Blue dyes. Conveniently label up to 100 μg of antibody per reaction, with 5 reactions per kit.
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Número de catálogoExcitación/emisiónColor
P30013410/455Pacific Blue
P30014405/551Pacific Orange
Número de catálogo P30013
Precio (USD)
-
Excitación/emisión:
410/455
Color:
Pacific Blue
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Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 μg per reaction). Each kit contains enough dye for five reactions.

A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Primary antibodies of the same isotype or derived from the same species can be multiplexed if they are directly labeled with compatible fluorophores. Using Pacific Blue and Pacific Orange dyes simultaneously allows you to excite both dyes at the same wavelength while their emissions are differentially distinguished.

Pacific Orange and Pacific Blue antibody labeling kits allow the labeling of small amounts of antibody with a violet light-excitable dye (up to 100 μg of antibody per reaction). Small amounts of other proteins (>40 kDa) can also be labeled using these kits.

Features of these antibody labeling kits
• Pacific Blue excitation and emission maxima: 410/455 nm
• Pacific Orange excitation and emission maxima: 405/551 nm
• Labeled proteins typically ready to use in 90 min. (∼15 min. hands-on time)
• Optimized for small-scale labeling of any protein >40 kDa
• Stabilizing proteins should be removed from the sample before labeling
• Purified using convenient spin filters
• Includes detailed instructions for determining degree of labeling (DOL)

These kits contain everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISA, immunocytochemistry, indirect FISH, and more. We are ISO 13485:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
ColorPacific Blue
Método de detecciónFluorescencia
Excitación/emisión410/455
Tipo de etiquetaColorantes Pacific
Escala de etiquetado100 μg
Línea de productosPacific Blue™
Tipo de productoKit de etiquetado de anticuerpos
Cantidad5 reacciones
Condiciones de envíoTemperatura ambiente
Reactividad químicaAmina
Labeling TargetAnticuerpos, Proteínas
Etiqueta o tintePacific Blue
SolubilidadDMSO (dimetilsulfóxido)
Unit SizeEach
Contenido y almacenamiento
Contiene cinco viales de colorante reactivo Pacific Blue™, un vial de bicarbonato sódico (84 mg), resina de purificación (10 ml), cinco columnas de centrifugación y cinco tubos de recogida.

Almacenar a entre 2 y 8 °C, protegido de la luz. El componente A puede almacenarse a entre -20 °C y 8 °C.
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Antibody Labeling Kits
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Figuras

Espectros de fluorescencia

Fluorescence spectra

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Documentos y descargas

Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3183146Certificate of Analysis17 jun 2025P30014
3086983Certificate of Analysis26 nov 2024P30013
2790065Certificate of Analysis20 jun 2024P30014
2892722Certificate of Analysis05 abr 2024P30013
2622321Certificate of Analysis06 oct 2023P30014
Se muestran 5 resultados, busque arriba un certificado específico

Hojas de datos de seguridad

Preguntas frecuentes

To allow for good reaction kinetics, antibodies should be in PBS buffer at a concentration of 0.5-3.0 mg/ml. The antibody must be free of preservatives (azide etc.), amine containing buffers and carrier proteins such as BSA.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Degree of labeling (DOL) describes the number of fluorophores per antibody. For in vivo labeling experiments, the DOL is restricted to a narrow range because it has significant consequences for the biodistribution and clearance of the probe. For example, for in vivo imaging, we have determined that the DOL range for the far-red Alexa Fluor dyes is 1.5 to 3 molecules per antibody for optimal optical in vivo imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (3)

Citations & References
Abstract
Lytic immune synapse function requires filamentous actin deconstruction by Coronin 1A.
Authors:Mace EM, Orange JS,
Journal:
PubMed ID:24760828
'Lytic immune effector function depends upon directed secretion of cytolytic granules at the immunological synapse (IS) and requires dynamic rearrangement of filamentous (F)-actin. Coronin 1A (Coro1A) is the hematopoietic-specific member of the Coronin family of actin regulators that promote F-actin disassembly. Here, we show that Coro1A is required for natural ... More
Perinucleolar relocalization and nucleolin as crucial events in the transcriptional activation of key genes in mantle cell lymphoma.
Authors:Allinne J, Pichugin A, Iarovaia O, Klibi M, Barat A, Zlotek-Zlotkiewicz E, Markozashvili D, Petrova N, Camara-Clayette V, Ioudinkova E, Wiels J, Razin SV, Ribrag V, Lipinski M, Vassetzky YS,
Journal:
PubMed ID:24452204
'In mantle cell lymphoma (MCL), one allele of the cyclin D1 (Ccnd1) gene is translocated from its normal localization on chromosome 11 to chromosome 14. This is considered as the crucial event in the transformation process of a normal naive B-cell; however, the actual molecular mechanism leading to Ccnd1 activation ... More
PLC-gamma2 is essential for formation and maintenance of memory B cells.
Authors:Hikida M, Casola S, Takahashi N, Kaji T, Takemori T, Rajewsky K, Kurosaki T,
Journal:J Exp Med
PubMed ID:19273623
Resting antigen-experienced memory B cells are thought to be responsible for the more rapid and robust antibody responses after antigen reencounter, which are the hallmark of memory humoral responses. The molecular basis for the development and survival of memory B cells remains largely unknown. We report that phospholipase C (PLC) ... More
3 total citations

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