Gentamicin/Amphotericin Solution
Gentamicin/Amphotericin Solution
Gibco™

Gentamicin/Amphotericin Solution

Una solución concentrada (500×) y estéril de antibiótico⁄antimicótico de gentamicina y anfotericina B (10 viales de 1 ml⁄envase).× Un paqueteMás información
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Número de catálogoCantidad
R0151010 x 1 mL
Número de catálogo R01510
Precio (USD)
-
Cantidad:
10 x 1 mL
Una solución concentrada (500×) y estéril de antibiótico⁄antimicótico de gentamicina y anfotericina B (10 viales de 1 ml⁄envase).× Un paquete de 10 soluciones de gentamicina⁄anfotericina es suficiente para complementar 10 frascos de 500 ml de medio basal. Libre de origen animal.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración500 X, 125 μg/mL Amphotericin B, 5 mg/mL Gentamicin
Línea de productosCascade Biologics™
Almacenamiento recomendado

menos 20 grados C

Condiciones de envíoHielo seco
Filtrado estérilYes
Aplicación validadaPrevención de la contaminación del cultivo celular
Forma físicaLíquido
Cantidad10 x 1 mL
TipoAnfotericina B, gentamicina
Unit SizeEach

Preguntas frecuentes

What alternative product do you offer for Gentamicin/Amphotericin Solution (Cat. No. R01510)?

We recommend ordering Gentamicin (10 mg/mL) (Cat. No. 15710064, 15710072) and Amphotericin B (250 µg/mL) (Cat. No. 15290026, 156290018) and mixing them together 1:1 to get a similar product as Gentamicin/Amphotericin Solution (Cat. No. R01510).

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Citations & References (7)

Citations & References
Abstract
Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.
Authors:Raffa P, Scattolini V, Gerli MFM, Perin S, Cui M, De Coppi P, Elvassore N, Caccin P, Luni C, Urciuolo A
Journal:Stem Cells Transl Med
PubMed ID:32578968
'Skeletal muscle decellularization allows the generation of natural scaffolds that retain the extracellular matrix (ECM) mechanical integrity, biological activity, and three-dimensional (3D) architecture of the native tissue. Recent reports showed that in vivo implantation of decellularized muscles supports muscle regeneration in volumetric muscle loss models, including nervous system and neuromuscular ... More
In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure.
Authors:Arnette C, Koetsier JL, Hoover P, Getsios S, Green KJ
Journal:Methods Enzymol
PubMed ID:26778564
'Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D ... More
Low-level arsenic causes proteotoxic stress and not oxidative stress.
Authors:Dodson M, de la Vega MR, Harder B, Castro-Portuguez R, Rodrigues SD, Wong PK, Chapman E, Zhang DD
Journal:Toxicol Appl Pharmacol
PubMed ID:29408041
'Prolonged exposure to arsenic has been shown to increase the risk of developing a number of diseases, including cancer and type II diabetes. Arsenic is present throughout the environment in its inorganic forms, and the level of exposure varies greatly by geographical location. The current recommended maximum level of arsenic ... More
Erratum: Long-range self-organization of cytoskeletal myosin II filament stacks.
Authors:Hu S, Dasbiswas K, Guo Z, Tee YH, Thiagarajan V, Hersen P, Chew TL, Safran SA, Zaidel-Bar R, Bershadsky AD
Journal:Nat Cell Biol
PubMed ID:28248306
Cholesterol crystals increase vascular permeability by inactivating SHP2 and disrupting adherens junctions.
Authors:Mani AM, Chattopadhyay R, Singh NK, Rao GN
Journal:Free Radic Biol Med
PubMed ID:29782988
To understand the adverse effects of cholesterol crystals on vascular homeostasis, we have studied their effects on endothelial barrier function. Cholesterol crystals increased endothelial barrier permeability in a dose and time dependent manner. In addition, cholesterol crystals induced tyrosine phosphorylation of VE-cadherin and a-catenin, disrupting endothelial AJ and its barrier ... More
7 total citations

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