The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
•Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters

The Collibri PCR-free ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library, which is enough for library quantification  and sequencing and multiple runs on Illumina instruments.

Applications
The Collibri PCR-free ES DNA Library Preparation Kit is recommended for:
• Whole genome sequencing
• Resequencing
• De novo assembly